ISSN:
0935-6304
Keywords:
Supercritical fluid chromatography
;
Capillary columns
;
Injection techniques
;
Reproducibility
;
Sensitivity
;
In-line dual detection
;
Explosives
;
Chemistry
;
Analytical Chemistry and Spectroscopy
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The reproducibility of peak areas as a function of the technique used for sample injection was investigated in capillary supercritical fluid chromatography (SFC). An injection technique has been developed to increase the volume of sample introduced into the capillary column. Using a modified time-split injection technique, long injection duration times were successfully applied to achieve lower detection limits. Analytes were effectively focused at the head of the analytical column using a unique pressure trap program. Because this on-column focusing was performed only by pressure and temperature programming, no instrumental modifications were necessary. Up to 1.0 μL of sample solution was injected onto 50 μm i.d. columns using this technique, with no observable peak splitting. Dual detection using ultraviolet (UV) absorption and flame ionization detection (FID) was performed in series, thereby avoiding the necessity of splitting the column effluent. For the compounds investigated (five nitroaromatics and one phthalate ester), the absolute sensitivity of the UV detector was significantly greater than that of the FID.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/jhrc.1240121008
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