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  • 1985-1989  (1)
  • 1970-1974  (2)
  • Ultrastructure  (2)
  • Cytokinesis  (1)
  • Elution  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Archives of dermatological research 281 (1989), S. 406-410 
    ISSN: 1432-069X
    Schlagwort(e): Dermatitis herpetiformis ; Immunoglobulins ; Complement components ; Elution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary To better understand the role of autoimmunity in the pathogenesis of dermatitis herpetiformis, linear IgA bullous dermatosis or other skin disorders, the antigenic specificity of the immune reactants bound in vivo in the skin must be identified. In order to do so, one must first be able to elute these immune reactants from the skin. We describe here a simple method of eluting not only specifically bound IgG, but also IgA and other immunoglobulins and complement components from skin biopsy material. The method involves cutaneous washing of the entrapped serum proteins in PBS pH 7 and pH 5 buffers followed by specific immunoglobulin elutions at pH 3 and 2. The IgA deposits which could not be removed by this treatment were eluted by a combination of low pH (0.5 M citrate pH 2) and a chaotropic agent (2 M NaCl). The relative concentration of IgA in eluates when quantitated by fluoroimmunoassay were three-to five-fold higher in dermatitis herpetiformis skin biopsy specimens, than in eluates of bullous pemphigoid or normal skin biopsy specimens.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 113 (1971), S. 388-395 
    ISSN: 1432-0878
    Schlagwort(e): Pomatoceros triqueter ; Egg investments ; Fertilization reaction ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ultrastructure of unfertilized and fertilized egg investments ofPomatoceros triqueter is described. A characteristic feature is the presence of a hitherto unrecorded additional investment layer, inserted between the chorion and the “outer border layer”, which is referred to as the intermediate layer. The fertilization reaction consists of the release of cortical granule material into the perivitelline “space”, and the severing of the microvillous cytosome to “outer border layer” connections, thus allowing an increase in the width of the perivitelline “space”. More ridged corrugations are found on the outer surface of the investments in the fertilized as compared with the unfertilized condition.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 123 (1972), S. 486-495 
    ISSN: 1432-0878
    Schlagwort(e): Pomatoceros triqueter ; Cytokinesis ; Surface folding ; New surface membrane ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The first cleavage division of Pomatoceros triqueter eggs is described. Time-lapse microcinematographic and electron microscopic studies revealed that, prior to division, the plasma membrane was folded into pleats. These were not present after division. This fact pointed to an unfolding of the plasma membrane which enabled it to cover the increased surface area resulting from cleavage. It is suggested that the pre-cleavage folds are derived from the membranes of the cortical granules, which continue releasing their contents into the perivitelline region following fertilization and first division. Filamentous material in the form of a band was present subjacent to the plasma membrane in the region of the furrow. The individual filaments of this band measure 5 to 7 nm in cross section, the dimensions being similar to those described for other kinds of dividing cells.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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