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  • 1985-1989  (1)
  • 1970-1974  (1)
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  • 1
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Colchicine induces the formation of small nuclei called micronuclei which contain limited parts of the genome. Some of them exhibit a DNA content equivalent to that of a single chromosome. Our purpose was to study the preservation of chromosome integrity during this micronucleation in PtK1 cells. Observation of karyotypes obtained after 3 days of cell cycle restoration revealed that micronucleation did not affect chromosome integrity or the presence of each chromosome pair in the surviving cells. In ‘early restoration’ cells, all the chromosomes included a centromere and were represented in the karyotype, but at variable rates. Furthermore, flow cytometry analysis of micronucleated cells, intermediate in DNA rate between control PtK1 cells in g1 and those in G2/M phases, led us to consider the possibility of selective replication of some chromosomes during micronucleation. Using antibodies against the kinetochore proteins, we derived the presence of one centromeric region (1–2 spots) in the smallest micronuclei. Therefore, these data (karyotypes, number of chromosomes, DNA content and kinetochore proteins) seem to indicate that micronucleation does not induce chromosome damages or translocations. Micronuclei are a convenient tool for investigation of the role of the different chromosomes in the organization of the interphase nuclei.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 148 (1974), S. 381-396 
    ISSN: 1432-0878
    Keywords: Placenta ; Mouse ; Syncytium ; Morphogenesis ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'origine embryologique des constituants cytologiques de la barrière placentaire chez la Souris a été mise en évidence du 8ème au 11éme jour de la gestation et la séquence des phénomènes favorisant la formation d'un syncytium trophoblastique, précisée. La barrière placentaire trilaminaire a une double origine: la couche interne provient du trophoblaste chorionique, la couche médiane syncytiale comme la couche externe dérivent du trophoblaste ectoplacentaire. Le stimulus initial de la différenciation semble résulter au 9ème jour du contact entre le trophoblaste chorionique et le mésoderme allantoïdien. Quelques heures après, on observe la réaction des cellules chorioniques, caractérisée par une augmentation importante des nucléoprotéines cytoplasmiques, puis l'apparition de globules lipidiques et enfin des propriétés de motilité. Ces cellules établissent des jonctions complexes avec le trophoblaste ectoplacentaire indifférencié qu'elles côtoient. En même temps, le métabolisme nucléaire de ces cellules ectoplacentaires semble modifié. Ultérieurement, au 10éme jour de la gestation, la disparition des membranes plasmiques latérales de ces cellules, conduit à la formation d'un syncytium par fusion de cellules au même stade d'évolution.
    Notes: Summary The morphogenesis of the different layers forming the mouse placental barrier is described during the 8th to 11th day of gestation. The timing and events leading to the formation of the syncytiotrophoblast are analyzed. Two different processes lead to the formation of the hemotrichorial placental membrane: the internal layer is formed by the chorionic trophoblast, both the intermediate syncytial layer and the external cytotrophoblast layer are derived from the ectoplacental trophoblast. The differentiation of the placental barrier begins on the 9th day of gestation by the establishment of contact between the chorionic trophoblast and the allantoic mesoderm. A few hours later, the chorionic cells show a considerable increase in cytoplasmic nucleoproteins and lipids and become ameboid. Complex cell-junctions differentiate between these chorionic cells and the adjacent ectoplacental trophoblast. On the 10th day of gestation the lateral cytoplasmic membranes disappear leading to the formation of a syncytium.
    Type of Medium: Electronic Resource
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