ISSN:
0884-3996
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
A novel optical biosensor for homogeneous immunoassay has been developed on the basis of the finding that electrochemical luminescence of pyrene-labelled antigen is extremely inhibited by immunochemical complexation. Electrochemical luminescence homogeneous immunoassay for human serum albumin (HSA), as a model analyte, was performed with a platinum plate electrode which was located in the vicinity of an optical fibre tip. HSA was determined in the concentration range of 3-25 × 10-6 mol/I.To improve electrochemical luminescence measurement an optical fibre electrode has been developed by fabricating a transparent platinum film on the top of an optical fibre. The minimum detectable limit of luminol was 10-11 mol/l with the optical fibre electrode. Luminol was applied as a label for homogeneous immunoassay.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bio.1170040170
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