ISSN:
1433-8580
Keywords:
Liver blood flow
;
Miniature pig
;
Indocyanine green
;
Indwelling catheters
;
Liver circulation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary For studies in physiology and biochemistry of the liver the measurement of hepatic blood flow is a fundamental precondition. Therefore, the least invasive method, the measurement of liver blood flow by indocyanine green (ICG) dye elimination, was re-evaluated for the miniature pig, an animal model of increasing interest. The accuracy of spectrophotometric determination of ICG was enhanced by addition of 5% of desoxycholic acid. It was shown that ICG was eliminated exclusively by the liver. An extraction rate of 30%–50% was found in mini-pigs, but an insufficient rate of 5%–10% in pigs of German landrace. A liver blood flow ranging from 70 to 160 ml/100g·min−1 was measured. These values are in good accordance with datas from simultaneous estimation of blood flow by133Xenon washout. As shown for a starvation/refeeding cycle, liver blood flow measurement by ICG combined with permanently implanted catheters is a practicable tool for studies of the metabolic and pharmacologic fate of substances resorbed from the gut.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01854971
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