ISSN:
1612-1112
Keywords:
Anion-exchange chromatography
;
Industrial-scale chromatography
;
Protein separation
;
Sepabeads FP series
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Sepabeads FP series with a variety of anion-exhanger type functional groups have been examined as largescale chromatographic separation media for proteins. The adsorption-desorption behavior of proteins has been found to be dependent mainly on the type and partly on the content of the attached amino ligands. The effect of the attached groups are discussed using batch measurement data. It has been found that the existence of ethanol residue on the nitrogen atom incrases the hydrophilic nature of quaternary ammonium type packings and thus gives a higher protein adsorption capacity. Among weakly basic type anionexchangers, polyethyleneimine-modified packings showed very large protein capacities.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02327665
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