ISSN:
1617-4623
Keywords:
Aspergillus nidulans
;
p-Fluorophenylalanine
;
Resistant mutants
;
Phe-tRNA synthetase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have isolated and characterized a new class of p-fluorophenylalanine (FPA)-resistant mutant in Aspergillus nidulans using a phenA strain as the wild type, by optimizing the conditions of growth. All four spontaneous mutants selected on a medium containing FPA were found to be recessive to their wild-type alleles in heterozygous diploids. Complementation analyses and linkage data showed that they were allelic and mapped at a single locus (fpaU) in the facA-riboD interval on the right arm of linkage group V. Partial purification and characterization of Phe-tRNA synthetase from wild-type and mutant strains revealed that the mutant enzyme had a greatly reduced ability to activate the analogue. It is suggested that mutation in the fpaU gene brings about a structural alteration in Phe-tRNA synthetase.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00329853
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