ISSN:
1432-2242
Keywords:
Parthenogenetic aphid populations
;
Allozymes
;
Polymorphic loci
;
Hidden genetic variation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A study of variation in three peptidases (PEP−3 to −5) in a parthenogenetic S. avenae field population at Rothamsted using serial one-dimensional polyacrylamide gel electrophoresis (involving changes of gel concentration and electrophoretic run-time) increased the overall number of “allozymes” (mobility variants) detected from 10 under standard conditions (6% gels, 2 h run-time) to 22, as well as revealing putative heterozygous banding patterns under some test conditions. However, an examination of another enzyme, 6-phosphogluconate dehydrogenase (6-PGD) in a sample collected at Rothamsted the following year failed, using a combination of serial methods (changes of gel concentration) and isoelectric focusing, to increase the total number of 6-PGD bands separated (seven, none of which appeared to be allelic in origin). Nevertheless, some major bands were split into several bands, whilst other infrequent bands were either gained or lost. The findings are briefly discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00273746
Permalink
