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  • 1985-1989  (2)
  • 1915-1919
  • Cell & Developmental Biology  (1)
  • Engineering  (1)
  • 1
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Optical indicators of the cationic, cyanine and anionic oxonol classes were used to evaluate the plasma membrane potential of animal cells in suspension and in monolayer culture. The optical signals were calibrated by using diffusion potential either of K+ (in the presence of valinomycin) or of H+ (in the presence of carbonyl cyanide p-trifluoromethoxyphenylhydrazone; FCCP); both classes of dye gave similar values of plasma membrane potential, in the range -40 to -90 mV for different cell types. Addition of haemolytic Sendai virus or Staphylococcus aureus α-toxin depolarizes cells and causes them to leak monovalent cations; these effects are antagonized by extracellular Ca2+. Cells infected with vesicular stomatitis or Semliki Forest virus become depolarized during an infectious cycle; infection with other viruses was without affect on plasma membrane potential.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Communications in Applied Numerical Methods 1 (1985), S. 113-121 
    ISSN: 0748-8025
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A traction boundary condition is particularly useful when analysing flow governed by the Navier-Stokes equation. For a large range of flow situations, such as fully developed laminar flow in a pipe or far field flow, the values of boundary traction can be evaluated explicitly. The former can be readily determined from the local values of the primitive variables, while the latter corresponds to zero traction. A general approach is advocated where traction boundary conditions can be evaluated as part of the solution procedure and its value is not required, a priori, before the solution can proceed. An iterative procedure for the evaluation and imposition of non-zero tractions on boundaries, where advection is appreciable, is demonstrated by utilizing the finite element method (FEM) to simulate laminar flow, both steady state and transient, rearward of a downstream-facing step within a bounded domain.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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