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Digitale Medien

A minimal error conjugate gradient method for ill-posed problems (1989)

King, J. T.

Springer

Journal of optimization theory and applications 60 (1989), S. 297-304

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ISSN: 1573-2878

Schlagwort(e): Conjugate gradient methods ; ill-posed problems ; first-kind operator equation ; minimal error

Quelle: Springer Online Journal Archives 1860-2000

Thema: Mathematik

Notizen: Abstract For the ill-posed operator equationTx=y in Hilbert space, we introduce a modification of the usual conjugate gradient method which minimizes the error, not the residual, at each step. Moreover, the error is minimized over the same finite-dimensional subspace that is associated with the usual method.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00940009

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Digitale Medien

An efficient procedure for isolation of nuclei from plant protoplasts (1985)

Saxena, Praveen K. ; Fowke, L. C. ; King, J.

Springer

Protoplasma 128 (1985), S. 184-189

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ISSN: 1615-6102

Schlagwort(e): Nuclear isolation ; pH ; Protoplasts ; Ultrastructure

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary The present communication describes an easy, efficient and rapid method for isolation of nuclei from plant protoplasts. Release of nuclei is accomplished by disruption of protoplasts in an appropriate buffer containing a very low concentration (0.01%) of the detergent Triton X-100. The pH of the nuclei isolation buffer (5.3) played a critical role in the recovery of stable nuclei in large numbers. Supplementation of buffer (10 mM MES) with spermine (0.1 mM), dithiothreitol (2.5 mM), ethylenediaminetetraacetic acid (2.5 mM) and Nad and KCl (10 mM each) improved nuclear yield and quality. With the method developed it is possible to routinely recover 95% nuclei from the protoplasts within 30 minutes. The nuclear preparations are of high purity with little detectable cytoplasmic contamination and no clumping of the nuclei. The structural integrity of the nuclei has been assessed and confirmed by Nomarski differential interference contrast optics and ultrastructural observations.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01276340

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