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  • 1985-1989  (2)
  • 1870-1879
  • Plant cell culture  (1)
  • Ultrastructure  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 417-422 
    Details
    ISSN: 1432-2242
    Keywords: Gene amplification ; Datura ; Sulfonylurea resistance ; Plant cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289814
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An efficient procedure for isolation of nuclei from plant protoplasts (1985)
    Springer
    Protoplasma 128 (1985), S. 184-189 
    Details
    ISSN: 1615-6102
    Keywords: Nuclear isolation ; pH ; Protoplasts ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The present communication describes an easy, efficient and rapid method for isolation of nuclei from plant protoplasts. Release of nuclei is accomplished by disruption of protoplasts in an appropriate buffer containing a very low concentration (0.01%) of the detergent Triton X-100. The pH of the nuclei isolation buffer (5.3) played a critical role in the recovery of stable nuclei in large numbers. Supplementation of buffer (10 mM MES) with spermine (0.1 mM), dithiothreitol (2.5 mM), ethylenediaminetetraacetic acid (2.5 mM) and Nad and KCl (10 mM each) improved nuclear yield and quality. With the method developed it is possible to routinely recover 95% nuclei from the protoplasts within 30 minutes. The nuclear preparations are of high purity with little detectable cytoplasmic contamination and no clumping of the nuclei. The structural integrity of the nuclei has been assessed and confirmed by Nomarski differential interference contrast optics and ultrastructural observations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276340
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    Paper (German National Licenses)
    Fulltext
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