ISSN:
1432-0428
Keywords:
Major histocompatibility complex
;
non-obese diabetic mouse
;
non-obese non-diabetic mouse
;
cataract Shionogi mouse
;
insulin-dependent diabetes
;
restriction fragment length polymorphisms
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary We have studied with a series of monoclonal antibodies and restriction fragment analysis the K, D, and class II region of the major histocompatibility complex of the non-obese diabetic mouse in comparison with its sister strains, the non-obese non-diabetic and cataract Shionogi mouse. (1) K region: Monoclonal antibody 31-3-4S (anti-Kd) reacted with splenocytes from non-obese diabetic mice while other anti-K (Kb, Kk, Kq) monoclonals did not react. Splenocytes from non-obese non-diabetic mice reacted with both anti-Kb and Kk monoclonals while splenocytes from cataract Shionogi mice reacted with anti-Kd and Kk monoclonals. Both sister strains, therefore, differ from the non-obese diabetic and other known mice strains by monoclonal analysis of H-2K. (2) D region: Splenocytes from both non-obese diabetic and non-obese non-diabetic mice reacted with monoclonal antibody 28-14-8S (anti-Db) while splenocytes from cataract Shionogi mice did not react with any anti-D monoclonal antibody tested. (3a) Class II region (non-obese diabetic and non-obese non-diabetic mice): Three of 11 monoclonal antibodies to class II molecules reacted with splenocytes of the non-obese diabetic mouse. The 3 reacting monoclonals have I-Ak primary specificities though additional anti-I-Ak monoclonal antibodies were negative. Among these monoclonals, 39B and 40A reacted with the non-obese diabetic mouse but not with the non-obese non-diabetic mouse, while 10-2-16 reacted with non-obese diabetic, non-obese non-diabetic and cataract Shionogi mice. Monoclonal MKD6 (anti-I-Ad) reacted with non-obese non-diabetic but not non-obese diabetic mice. In crosses of non-obese diabetic with non-obese non-diabetic mice, splenocytes from all diabetic backcrosses studied (6/6) were positive with monoclonal 40A but negative with MKD6 indicating that the major histocompatibility complex of non-obese non-diabetic mice is not diabetogenic and confirming major histocompatibility complex-linkage of the diabetogenic gene in this additional cross. (3b) Class II region (cataract Shionogi mice): Utilising 11 anti-class II monoclonal antibodies the pattern of reactivity of the cataract Shionogi mouse was identical to the non-obese diabetic mouse. Splenocytes from both non-obese diabetic and cataract Shionogi mice fail to express I-E (no reaction with monoclonal 14-4-4). In addition, using an I-A alpha probe with restriction endonuclease HindIII or I-A beta probe with BamHI, the restriction fragment length polymorphism pattern of the cataract Shionogi mouse was identical to the non-obese diabetic mouse. In summary, the cataract Shionogi mouse appears to have a similar I-A region to the non-obese diabetic mouse but differs at both the K and D region. With the hypothesis that the unique I-A beta of the non-obese diabetic mouse is diabetogenic, the cataract Shionogi mouse should provide a new strain for further characterisation of diabetogenic genes of the non-obese diabetic mouse since it is similar at I-A, fails to express I-E, but differs at both class I loci.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290594
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