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Fundamental conditions in pressure-programmed supercritical fluid chromatography-mass spectrometry and some applications to vitamin analysis (1986)

Matsumoto, K. ; Tsuge, S. ; Hirata, Y.

Springer

Chromatographia 21 (1986), S. 617-621

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ISSN: 1612-1112

Keywords: Supercritical fluid chromatography-mass spectrometry ; Pressure programming ; Analysis of vitamins

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary A combined system of pressure-programmed packed capillary supercritical fluid chromatography-mass spectrometry (SFC/MS) was constructed using a self-soupting interface assisted by vacuum nebulizing. For the optimum operation of the SFC/MS system, fundamental analytical conditions such as the flow rate of the mobile phase, the pump pressure and the composition of the mobile phase were examined. The use of large packing materials indicated that the capacity factor for a sample solute is almost constant under a given pump pressure regardless of the flow rate of the supercritical fluid. The SFC/MS system was applied to the analysis of both water- and fat-soluble vitamins. Both types vitamins were clearly separated under basically the same SFC conditions. High quality mass spectra of the vitamins were obtained; selected ion monitoring (SIM) traces of the vitamins are also reported as well as their UV traces.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02311914

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Production of identical twins by separating two-cell rat embryos (1989)

Matsumoto, K. ; Miyake, M. ; Utsumi, K. ; [et al.]

New York, NY : Wiley-Blackwell

Gamete Research 22 (1989), S. 257-263

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ISSN: 0148-7280

Keywords: micromanipulation ; uterine transfer ; oviduct transfer ; blastomeres ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology

Notes: Rat identical twins were produced from two-cell embryos. In the presence of cytochalasin B, rat two-cell embryos could be separated efficiently into two blastomeres by micromanipulation. Isolated blastomeres, embedded in agar cylinders and cultivated in ligated rat oviducts for 3 days, developed to the morula or blastocyst stage. After removing the agar, pairs of developed one-half embryos were transferred into Day 1 oviducts or Day 4 uteri of pseudopregnant rats. The percentage of embryos, separated either in the presence or absence of cytochalasin B, that developed into live fetuses was higher in cases of uterine transfer than in cases of oviduct transfer (38% vs. 18%, 31% vs. 15%, respectively). Throughout the present experiment, nine pairs of identical twins were successfully produced. This is the first report of the production of identical rat twins by separating two-cell embryos.

Additional Material: 4 Ill.