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  • 1985-1989  (2)
  • Asthma  (1)
  • Asthmatic children  (1)
  • Phenylephrine
  • Temperature
  • 1
    Electronic Resource
    Electronic Resource
    β-Adrenoceptor density and resolution of high and low affinity state on B- and T-cells in asthmatic and non-asthmatic children (1988)
    Springer
    European journal of pediatrics 147 (1988), S. 116-120 
    Details
    ISSN: 1432-1076
    Keywords: β-Adrenoceptor density ; β-Adrenoceptor affinity ; High and low receptor affinity state ; B and T-cells ; Asthmatic children
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract β-adrenoceptor binding in lymphocytes of asthmatic and non-asthmatic children and healthy adult volunteers was investigated with the radioligand 125-iodocyanopindolol (ICYP). Binding studies were performed with 4 to 5 different concentrations of ICYP. Receptor density and affinity were calculated by Scatchard plots. Resolution of β-adrenoceptors into those of high and low affinity state was obtained from inhibition curves with salbutamol using Hofstee plots. Receptor density in B-cell enriched fractions was two to three-fold higher than in T-cells for all patients and volunteers studied (P less than 0.025). No difference in β-adrenoceptor density on B and T-cells occurred neither in age-matched asthmatic and non-asthmatic children nor in adult volunteers. The affinity of β-adrenoceptors did not differ for B and T-cells nor for the patients or volunteers studied. However, when two distinct binding states for β-adrenoceptor agonists were obtained using salbutamol displacement curves it appeared that β-adrenoceptors on T-cells were at a higher affinity state compared to those on B-cells in asthmatic and non-asthmatic children, as well as in adults. Since the ability of an agonist to activate adenylate cyclase correlates closely with the amount of high affinity receptor state formed in the presence of the agonist, increased intrinsic activity of the β-adrenoceptor agonist on T-cells may be postulated. In conclusion, age-related control groups and determination of the B/T ratio are neccessary for interpretation of β-adrenoceptor changes in bronchial asthma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442206
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Glucocorticoid receptors in mononuclear blood cells and their correlation to endogenous and exogenous corticoids in healthy and asthmatic children (1988)
    Springer
    European journal of pediatrics 147 (1988), S. 490-495 
    Details
    ISSN: 1432-1076
    Keywords: Glucocorticoid receptors ; Asthma ; Prednisolone therapy ; Free serum and urine cortisol ; Children
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The number and affinity of glucocorticoid binding sites in peripheral mononuclear cells (MNC) of asthmatic and healthy children were determined by a whole cell (3H)dexamethasone binding assay at 37°C. Using HPLC determination, corresponding serum levels of non-protein-bound (free) cortisol, whole cortisol and cortisone as well as urine excretion of free cortisone and cortisol were assessed. The average number of binding sites (BS) per cell and the dissociation constant (KD) respectively, in atopic asthmatics (7768±666 BS/MNC resp. KD=17.2±2 nM) did not differ from the values measured in our control group (8333±691 BS/MNC resp. 25.4±4.8 nM). Within the age range 1 month-15.8 years neither age-dependent changes nor sex-related differences in the number of binding sites or the KD values could be detected. Active or currently inactive asthmatics, and patients under different antiasthmatic drug regimes, had similar binding sites on MNC. No differences in serum levels of cortisol, cortisone and free cortisol or in free cortisol and free cortisone of 24-h urine samples were found between healthy children and asthmatics. After a short course of prednisolone therapy for an acute severe asthmatic attack the number of glucocorticoid binding sites in peripheral MNC decreased to an average of 4632±421 BS/MNC, whereas the dissociation constant did not change significantly (14.5±3.6 nM). The corticod-hormone pattern in the serum, 24-h urine excretion, and the normal number and affinity of glucocorticoid receptors on peripheral MNC suggest that there is no primary, general impairment of glucocorticoid metabolism in asthmatic children. Short-term glucocorticoid administration resulted in suppression of endogenous corticoids to undetectable levels accompanied by down-regulation of glucocorticoid-receptor BS to about 55% of control levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441973
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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