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  • 1985-1989  (2)
  • Avena sativa  (1)
  • Ca current  (1)
  • Catecholamines
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 410 (1987), S. 385-393 
    ISSN: 1432-2013
    Keywords: Bovine aortic endothelial cells ; Patch clamp ; Inward rectifier ; Transient outward K current ; Ca current ; Endothelium-derived relaxing factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The electrophysiological properties of cultured bovine aortic endothelial cells were characterized using the patch clamp technique. Resting potentials were measured on passing to the whole cell recording configuration and were close to −65 mV in healthy cells. In cell-attached recordings with a high potassium pipette solution, inward single channel currents were observed with zero applied pipette potential. A linear slope conductance of 25 pS was found for a wide range of hyperpolarizing patch potentials and also for depolarizing patch potentials of up to 50–60 mV. A pronounced inward rectification was apparent as no reversal of these currents was seen for larger depolarizations. Whole cell recording in physiological solutions revealed the presence of a hyperpolarization-activated inward current with strong inward rectification and no voltage-dependent ionic current was observed upon depolarization in this subset of cells. Substitution of potassium for external sodium resulted in a shift in the zero current potential consistent with potassium being the main permeant ion. Together with the characteristic voltage-dependent blocking actions of external sodium ions and low concentrations of barium and caesium ions, our results indicate that this current is very similar to the classical inward rectifier as originally described in skeletal muscle and in tunicate eggs. In a second population of cells, a depolarization-activated outward current displaying characteristics of the fast transient A-type potassium current as first reported in molluscan neurones was also observed. No evidence for inward voltage dependent sodium or calcium currents was found.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5060
    Keywords: Avena sativa ; oats ; selection ; independent culling ; harvest index ; vegetative growth index ; unit straw weight ; biological yield ; growth rate ; heritability ; genotypic correlatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Most variation in grain yield of oats is due to variation in harvest index and vegetative growth index, but the latter traits are negatively associated. Therefore we used independent culling levels to select oat genotypes with high levels of vigor traits and a desirable level of harvest index in an attempts to maximize grain yield. Harvest index and vegetative growth index or harvest index and unit straw weight were selected at various culling levels. Intensive selection for harvest index resulted in high harvest index but no grain yield improvement, because the selected lines had poor vigor. Intensive selection for vegetative growth index or unit straw weight resulted in high biomass but low harvest index. The most effective combination of culling levels was to select 25% of the original population for harvest index and, subsequently, to select for vegetative growth index or unit straw weight at an 8% intensity in the remnant population.
    Type of Medium: Electronic Resource
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