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  • 1
    Electronic Resource
    Electronic Resource
    Haptoglobin phenotyping by polyacrylamide gel isoelectric focusing and its application to simultaneous typing of serum proteins (1988)
    Springer
    International journal of legal medicine 101 (1988), S. 37-40 
    Details
    ISSN: 1437-1596
    Keywords: Haptoglobin typing, polyacrylamide gel isoelectric focusing ; Blood groups, HP-phenotyping ; Haptoglobin-Typisierung, Isoelektrofokussierung in Polyacrylamidgel ; Blutgruppen, HP-Typisierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird eine einfache Methode zur Typisierung von Haptoglobin (HP) mittels Isoelektrofokussierung beschrieben. Das Serum wird mit Clostridium perfringens Neuraminidase (CPN), sodann mit Dithiothreitol (DTT) vorbehandelt und anschließend der Polyacrylamidgel-Isoelektrofokussierung (PAGIF) unterworfen. Die Banden wurden durch Immunoblotting sichtbar gemacht. Die Methode erwies sich als erfolgreich bei der HP-Darstellung von selbst 2 Monate alten Blutspuren. Durch eine leichte Modifizierung ist es möglich, neben HP auch die Komplement-Komponente C81 und Faktor I (IF) darzustellen. Immunoblotting ergab außerdem eine dauerhafte Darstellung der HP-Banden. PAGIF ist daher als forensische Routinemethode zur HP-Typisierung geeignet.
    Notes: Summary A simple isoelectric focusing method for haptoglobin (HP) typing is described. Serum was pretreated first with C. perfringens neuraminidase (CPN) and then with dithiothreitol (DTT). The treated serum was subjected to polyacrylamide gel isoelectric focusing (PAGIF), and the band patterns were detected by immunoblotting. The method could be successfully applied to HP typing of bloodstains as old as 2 months. A slight modification of it enabled HP, complement component C81, and factor I (IF) to be typed simultaneously. The immunoblotting facilitated preservation of HP patterns. Thus, the PAGIF method for HP typing is suitable for routine use in the forensic laboratory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205322
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Cell proliferation and cell loss in intramucosal signet ring cell carcinoma of canine stomachs induced by N-ethyl-N′-nitro-N-nitrosoguanidine (1985)
    Springer
    Journal of cancer research and clinical oncology 110 (1985), S. 87-94 
    Details
    ISSN: 1432-1335
    Keywords: Stomach ; Signet ring cell carcinoma ; Cell turnover ; Tritiated thymidine autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Signet ring cell carcinoma was induced in canine stomachs by N-ethyl-N′-nitro-N-nitrosoguanidine, and modes of cell proliferation and turnover in the carcinoma were studied by 3H-thymidine autoradiography in conjunction with morphometric analysis. From 2 to 15 months after the cessation of 8 months carcinogen treatment, carcinomas in an early stage were obtained. Most of the cancer tissues confined to the lamina propria showed a layered structure. This comprised three layers; the superficial and the deep layer were composed of signet ring cells, and the middle layer was composed of small round cells. The dogs were labeled with 3H-thymidine by s.c. injection and by local infusion of the celiac artery. Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Using a pulse labeling experiment, those labeled carcinoma cells were shown to migrate from the middle layer towards the surface. Morphometric analysis of the autoradiographs showed that the small cells in the middle layer migrated upwards and produced mucin to become full-blown signet ring cells by 5.5 days. In 15 days, most labeled cancer cells in the superficial layer had disappeared. This mode of cellular turnover appeared to mimic a cell renewal system of the normal gastric mucosa. If the cancer cells turn over in this way, the tumor must grow slowly, remaining as an intramucosal cancer for a relatively long period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402717
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  • 3
    Electronic Resource
    Electronic Resource
    Binding characteristics of ionic surfactants with human hair (1986)
    Springer
    Colloid & polymer science 264 (1986), S. 798-802 
    Details
    ISSN: 1435-1536
    Keywords: Binding isotherms ; ionic surfactants ; human hair ; thermodynamic parameReferences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Binding isotherms of two types of ionic surfactants, CmH2m +1SO4Na (m = 8,10,12) and CnH2n + 1N+(CH3)3C1 (n=10, 12), to human hair in aqueous solutions were examined to clarify effects of hydrophobic and electrostatic interaction of ionic surfactants with hair. The binding isotherms of anionic surfactants showed cooperativity with discontinuously increasing shapes, while the binding isotherms of cationic surfactants showed a Langmuir-type, regardless of the difference of a hair condition. The calculated free energy change (— ΔG@#@) for binding, obtained from Klotz' plots, suggests that the binding processes are governed mainly by a hydrophobic interaction, and bound surfactants probably expose their alkyl chains to the aqueous phase, since no-ΔG was observed with the increase of m or n and values of enthalpy change(ΔH) were positive or zero.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01500756
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    Paper (German National Licenses)
    Fulltext
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