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  • 1985-1989  (7)
  • Urinary tract repair  (5)
  • Biochemistry and Biotechnology  (1)
  • Calcium current  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The in vitro assessment of a collagen/vicryl (polyglactin) composite film together with candidate suture materials for use in urinary tract surgery (1987)
    Springer
    Urological research 15 (1987), S. 53-59 
    Details
    ISSN: 1434-0879
    Keywords: Collagen vicryl composite ; Urinary tract repair ; Sutures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A Vicryl (Polyglactin)/Collagen composite membrane has been developed for potential use in urinary tract surgery. The compatability of this membrane, together with its two individual components, collagen film and vicryl mesh, has been tested over a three week period in urine obtained from both healthy and from stone forming patients. The rate of degradation as indicated by changes in the mechanical strength was determined at regular intervals. In addition,absorbable suture materials such as plain catgut, chromic catgut, and vicryl were similarly tested; in the latter case, the breaking stress, and actual loss of weight of the material were compared. The whole series of experiments were then repeated in urine obtained from rabbits, the animal chosen for any future in vivo studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256337
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The use of a biodegradable collagen/vicryl composite membrane to repair partial nephrectomy in rabbits (1987)
    Springer
    Urological research 15 (1987), S. 239-242 
    Details
    ISSN: 1434-0879
    Keywords: Collagen vicryl composite ; Partial nephrectomy ; Urinary tract repair ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A composite membrane produced from collagen and vicryl mesh has been used to cover the kidney surface following partial nephrectomy in rabbits. The membrane readily held sutures, gave satisfactory haemostasis, and prevented leakage of urine. The experiments showed that the prosthesis biodegraded in less than twenty weeks. The only observed long-term effect of the material was thickening of the renal capsule. The results indicate that this membrane may be a suitable reparative materal for use in traumatised kidneys in humans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262107
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The in-vitro assessment of a collagen/vicryl (polyglactin) composite film together with candidate suture materials for use in urinary tract surgery (1988)
    Springer
    Urological research 16 (1988), S. 111-117 
    Details
    ISSN: 1434-0879
    Keywords: Collage vicryl composite ; Urinary tract repair ; Urinary calculi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Collagen coated vicryl mesh has been incubated with a series of urine collections from healthy and stoneforming patients. For comparison, collagen film, vicryl mesh, and a number of absorbable and non-absorbable sutures were similarly tested. Incubation in rabbit urine were also included in the study. Deposition of urinary salts, estimated qualitatively by electron microcopy, were observed on the collagen vicryl composite in approximately two thirds of the urines tested including rabbit urine. Those urines from patients with a high calcium excretion in particular caused urinary deposits on the material. Similar results were obtained with collagen film although the latter was not tested in rabbit urine. Considerably less deposits of urinary constituents were found with other absorbable materials such as vicryl mesh, vicryl sutures, and chromic catgut, whereas a higher proportion of concretions were found with the nonabsorbable sutures. The results indicate that urinary salt deposition may be a problem associated with collagen based composite materials after prolonged exposure to urine. It must, however, be emphasised that great care should be exercised when extrapolating any results obtained in-vitro to the in-vivo situation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261967
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The in-vitro assessment of a Collagen/Vicryl (Polyglactin) composite film together with candidate suture materials for potential use in urinary tract surgery (1988)
    Springer
    Urological research 16 (1988), S. 381-384 
    Details
    ISSN: 1434-0879
    Keywords: Collagen Vicryl composite ; Urinary tract repair ; Urinary tract infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A Collagen/Vicryl (Polyglactin) composite membrane (developed for use in urinary tract surgery) has been incubated in cultures of radioactively labelled urinary tract pathogens vis Escherichia coli, Staphylococcus epidermidis, and Proteus mirabilis for up to 1h. For comparison, collagen film, Vicryl mesh, and a number of absorbable and nonabsorbable sutures were similarly tested. Following incubation, samples were also examined by scanning electron microscopy. Under the experimental conditions employed, only minimal adherence of the micro-organisms to the collagen coated Vicryl mesh, its two individual components, as well as Vicryl and nylon sutures was observed. Significantly greater numbers of bacteria, however, ahered to silk and Chromic Catgut. The results suggest that adherence of microorganisms to the prosthesis even in infected urine is unlikely to develop into a microcolony of bacteria. However, it should be emphasised that great care must be excercised when extrapolating from the in-vitro to the in-vivo situation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00256047
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  • 5
    Electronic Resource
    Electronic Resource
    An assessment of a collagen/vicryl composite membrane to repair defects of the urinary bladder in rabbits (1987)
    Springer
    Urological research 15 (1987), S. 235-238 
    Details
    ISSN: 1434-0879
    Keywords: Bladder ; Urinary tract repair ; Collagen/vicryl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Collagen/vicryl (Polyglactin) composite membrane has been used to repair full-thickness defects in the urinary bladder of rabbits. The material has been shown to be biodegradable, prevent leakage of urine, and is readily replaced by collagenous scar tissue lined with a urothelium. Regeneration of smooth nuscle has been observed in the repair area of some animals. The results suggest that such a material may well be of use to urologists wishing to augment contracted bladders or in the repair of bladder fistulae in human subjects, thereby avoiding the use of bowel or other material e.g. omentum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262106
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Photoactivation of intracellular guanosine triphosphate analogues reduces the amplitude and slows the kinetics of voltage-activated calcium channel currents in sensory neurones (1988)
    Springer
    Pflügers Archiv 411 (1988), S. 628-636 
    Details
    ISSN: 1432-2013
    Keywords: Guanine nucleotide analogue ; Calcium current ; Flash photolysis ; Guanine nucleotide binding protein ; dorsal root ganglion neurone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The influence of guanine nucleotide analogues on calcium channel currents in cultured rat dorsal root ganglion neurones has been studied using a technique in which the rate of diffusion of the analogues to their site of action is by-passed by photochemical release of the analogues within the neurones. The 1(2-nitrophenyl)ethyl P3-ester derivatives of guanosine 5′-0(3-thio)triphosphate (caged GTP-γ-S) and 5′-guanylylimidodiphosphate (caged GMP-PNP) were synthesised and found to be completely photolysable by light, yielding free GTP-γ-S and GMP-PNP. Calcium channel currents were recorded using the whole cell patch technique and either caged GTP-γ-S or caged GMP-PNP (2 mM) were included in the patch pipette. Stable currents were recorded for 5–10 min, and a single pulse of 300–350 nm irradiation was directed using a liquid light guide onto the recording dish. Calcium channel currents were then recorded every 30–120 s following photochemical release of approximately 20μM GTP-gg-S. The peak calcium channel current was reduced by about 70% with a slow time course [t 1/2 1.5±0.2 min (mean±SEM);n=5]. The transient component of the peak current was usually completely abolished, whereas the sustained current measured at the end of the 100 ms depolarising pulse was less affected. Qualitatively similar effects were observed on photolysis of caged GMP-PNP. These results suggest that the channels underlying the transient and the sustained components of the whole cell current may be differentially molulated by GTP analogues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00580858
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Single photon counting imagery (1989)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 4 (1989), S. 507-511 
    Details
    ISSN: 0884-3996
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Advent of the multichannel plate and position sensitive detector has made possible true single photon counting imaging tubes. We have investigated the application of these detectors in studies of the ultraweak light emission of biological materials. Initially, we focussed our efforts on two objectives: (1) obtaining single photon counting images of living tissues using only the light (chemiluminescence) emitted by the specimen and (2) developing means of obtaining well-resolved spectra of weakly emitting sources. We have obtained a variety of images. One striking result of this work is the first observation of tissue specific localization of photon emission in situ. Using this detector we have also obtained the first well-resolved spectra of some important ultraweak emission processes. These results illustrate the potential use of single photon imaging in bioluminescence and chemiluminescence research.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170040166
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    Paper (German National Licenses)
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