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Separation and determination of nitrite ion by affinity chromatography with cyano(pheophytinato a)cobalt(III) charged column (1989)

Furuya, K. ; Hatano, H. ; Inoue, H. ; [et al.]

Springer

Chromatographia 27 (1989), S. 461-464

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Chlorophyll-bonded stationary phase ; Cyano(pheophytinato a)cobalt(III) ; Pheophytin a

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary The separation and determination of the nitrite ion by affinity chromatography is described. The good separation of the nitrite ion was obtained by using cyano-(pheophytinato a)cobalt(III) bonded octadecylsilica as the stationary phase and water of pH 4.3 adjusted by acetic acid as the mobile phase. Conventional ion chromatograph was also used for the analysis of the nitrite ion. For the synthetic samples prepared by mixing the nitrite and nitrate ions, analytical values obtained by affinity chromatography were in agreement with calculated values and close to the analytical values obtained by ion chromatography. In the proposed affinity chromatography, the nitrite and nitrate ions could be determined in the concentration range of 0.15–1.0ppm and 0.1–1.0ppm with relative standard deviations (n=10) of 5.3% and 4.7%, respectively.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02319564

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Effect of organic solvents on the activity of glucoamylase (1986)

Nagamoto, H. ; Yasuda, T. ; Inoue, H.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 28 (1986), S. 1172-1177

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ISSN: 0006-3592

Keywords: Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Hydrolyses of maltose, maltotriose, and soluble starch catalyzed by glucoamylase (Asp. Niger) were carried out in the aqueous solutions of methanol, ethanol, ethylene glycol, and 1,4-dioxane at 40°C and at the optimum pH in the respective solutions. By the kinetic analysis based on the subsite model, it was shown that the intrinsic rate constant, Kint, was electrostatically affected by the dielectric constant of the hydroorganic solutions. The affinity of the third subsite, A3, which affects the apparent rate constant, K0, was correlated with the ×Gtr's of maltose and amino acid side chains.

Additional Material: 5 Ill.