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  • 1
    Electronic Resource
    Electronic Resource
    Simultaneous Phenotyping of Genetic Markers for Paternity Testing (1987)
    Springer
    International journal of legal medicine 99 (1987), S. 135-142 
    Details
    ISSN: 1437-1596
    Keywords: Simultaneous phenotyping, genetic markers ; Isoelectric focusing, electrophoresis ; Immunoblotting, paternity testing ; Phänotypisierung genetischer Marker ; Vaterschaftsbegutachtung, kostensparende Methoden und Dokumentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es werden zeit- und kostensparende Methoden für die Vaterschaftsbegutachtung beschrieben. Siebzehn genetische Systeme werden in sechs Gruppen unterteilt. 1. Gruppe: Transferrin (Tf), Faktor B (BF) und Phosphoglucomutase 1 (PGM1); 2. Gruppe: Gc-System (Gc) oder α1-Antitrypsin (PI) und α-2HS-Glycoprotein (HSGA); 3. Gruppe: Komplement-Komponente C6 und C7, Faktor 13B (F13B) und Plasminogen (PLG); 4. Gruppe: Haptoglobin (Hp), C8 α-γ Kette (C81) und Faktor I (IF); 5. Gruppe: saure Erythrozyten-Phosphatase (ACP), Esterase D (ESD), Glutamat-Pyruvat-Transaminase (GPT); 6. Gruppe: 6-Phosphogluconat Dehydrogenase (PGD) und Glyoxalase I (GLO). Jede Gruppe wurde gleichzeitig mittels Elektrophorese oder isoelektrische Fokussierung (IEF) mit Färbung oder Immunoblotting untersucht. Diese Methoden erwiesen sich in der Praxis als zeit- und kostensparend und erleichtern die vorübergehende Aufbewahrung und Dokumentation der Elektrophorese-Bilder.
    Notes: Summary Time- and cost-saving methods for paternity testing are described. Seventeen genetic systems were divided into six groups: (1) transferrin (Tf), factor B (Bf), and phosphoglucomutase 1 (PGM1); (2) group-specific component (Gc) or α1-antitrypsin (PI) and α2HS-glycoprotein (HSGA); (3) complement components C6 and C7, factor 13B (F13B), and plasminogen (PLG); (4) haptoglobin (Hp), C8 α-γ chain (C81), and factor I (IF); (5) red cell acid phosphatase (ACP), esterase D (ESD), and glutamic-pyruvic transaminase (GPT); and (6) 6-phosphogluconate dehydrogenase (PGD) and glyoxalase I (GLO). Each group of systems was typed simultaneously by electrophoresis or isoelectric focusing (IEF) followed by staining or immunoblotting. These methods are very practical because they afford a considerable saving of time, work and expense, and facilitate semipermanent preservation of electrophoretic patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200633
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Haptoglobin phenotyping by polyacrylamide gel isoelectric focusing and its application to simultaneous typing of serum proteins (1988)
    Springer
    International journal of legal medicine 101 (1988), S. 37-40 
    Details
    ISSN: 1437-1596
    Keywords: Haptoglobin typing, polyacrylamide gel isoelectric focusing ; Blood groups, HP-phenotyping ; Haptoglobin-Typisierung, Isoelektrofokussierung in Polyacrylamidgel ; Blutgruppen, HP-Typisierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird eine einfache Methode zur Typisierung von Haptoglobin (HP) mittels Isoelektrofokussierung beschrieben. Das Serum wird mit Clostridium perfringens Neuraminidase (CPN), sodann mit Dithiothreitol (DTT) vorbehandelt und anschließend der Polyacrylamidgel-Isoelektrofokussierung (PAGIF) unterworfen. Die Banden wurden durch Immunoblotting sichtbar gemacht. Die Methode erwies sich als erfolgreich bei der HP-Darstellung von selbst 2 Monate alten Blutspuren. Durch eine leichte Modifizierung ist es möglich, neben HP auch die Komplement-Komponente C81 und Faktor I (IF) darzustellen. Immunoblotting ergab außerdem eine dauerhafte Darstellung der HP-Banden. PAGIF ist daher als forensische Routinemethode zur HP-Typisierung geeignet.
    Notes: Summary A simple isoelectric focusing method for haptoglobin (HP) typing is described. Serum was pretreated first with C. perfringens neuraminidase (CPN) and then with dithiothreitol (DTT). The treated serum was subjected to polyacrylamide gel isoelectric focusing (PAGIF), and the band patterns were detected by immunoblotting. The method could be successfully applied to HP typing of bloodstains as old as 2 months. A slight modification of it enabled HP, complement component C81, and factor I (IF) to be typed simultaneously. The immunoblotting facilitated preservation of HP patterns. Thus, the PAGIF method for HP typing is suitable for routine use in the forensic laboratory.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205322
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Cell proliferation and cell loss in intramucosal signet ring cell carcinoma of canine stomachs induced by N-ethyl-N′-nitro-N-nitrosoguanidine (1985)
    Springer
    Journal of cancer research and clinical oncology 110 (1985), S. 87-94 
    Details
    ISSN: 1432-1335
    Keywords: Stomach ; Signet ring cell carcinoma ; Cell turnover ; Tritiated thymidine autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Signet ring cell carcinoma was induced in canine stomachs by N-ethyl-N′-nitro-N-nitrosoguanidine, and modes of cell proliferation and turnover in the carcinoma were studied by 3H-thymidine autoradiography in conjunction with morphometric analysis. From 2 to 15 months after the cessation of 8 months carcinogen treatment, carcinomas in an early stage were obtained. Most of the cancer tissues confined to the lamina propria showed a layered structure. This comprised three layers; the superficial and the deep layer were composed of signet ring cells, and the middle layer was composed of small round cells. The dogs were labeled with 3H-thymidine by s.c. injection and by local infusion of the celiac artery. Flash-labeled autoradiographs revealed that most 3H-thymidine incorporating cancer cells were located around the middle layer, with a small amount of mucin. Using a pulse labeling experiment, those labeled carcinoma cells were shown to migrate from the middle layer towards the surface. Morphometric analysis of the autoradiographs showed that the small cells in the middle layer migrated upwards and produced mucin to become full-blown signet ring cells by 5.5 days. In 15 days, most labeled cancer cells in the superficial layer had disappeared. This mode of cellular turnover appeared to mimic a cell renewal system of the normal gastric mucosa. If the cancer cells turn over in this way, the tumor must grow slowly, remaining as an intramucosal cancer for a relatively long period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402717
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    Paper (German National Licenses)
    Fulltext
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