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Frequency distribution of plasma cells in the medullary cords of tumour-draining axillary and paracolic lymph nodes (1987)

Horst, Heinz-August ; Horny, Hans-Peter

Springer

Virchows Archiv 411 (1987), S. 239-243

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ISSN: 1432-2307

Keywords: Plasma cells ; Axillary lymph nodes ; Paracolic lymph nodes ; Medullary cords ; Breast cancer ; Adenocarcinoma of the large bowel

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary Five hundred and ninety-seven axillary lymph nodes draining 104 invasive ductal breast cancers, and 94 paracolic lymph nodes draining 30 invasive adenocarcinomas of the large bowel were investigated immunohistologically to determine the frequency distribution of plasma cells (PC) in the medullary cords (MC). The degree of plasmacytic infiltration was calculated semiquantitatively using the 3-grade scale (0/+, ++, +++) of Cottier et al. (1973). Statistical analysis yielded the following results: While a marked reactive plasmacytosis (+++) was seen in 28.7% of the paracolic lymph nodes, only 1.5% of the axillary lymph nodes exhibited a comparable degree of plasmacytic infiltration (p〈0.0001). Conversely, low PC counts (0/+) were encountered in 51.1% of the paracolic lymph nodes, but in 83.9% of the axillary lymph nodes. A comparison of axillary lymph nodes with and without nodal metastastation revealed significant differences (nodal-negative cases: 0/+: 83.6%, ++: 14.3%, +++: 2.1%; nodal-positive cases: 0/+: 84.3%, ++: 14.9%, +++: 0.8%). However, significantly more (p〈0.001) paracolic lymph nodes of the nodal-negative group revealed a marked plasmacytosis, whereas in the nodal-positive group lymph nodes with low PC counts were more frequent (nodal-negative cases: 0/+: 27.7%, ++: 29.7%, +++: 42.6% ; nodal-positive cases: 0/+: 74.5%, ++: 10.6%, +++: 14.9%). The degree of plasmacytic reactions in the tumour-regional lymph nodes was not related to the stage of the primary tumour. Moreover, no correlation exists between the PC content of the MC and the amount of PC in metastatic deposits of the same lymph nodes. Altogether, these results do not support the concept that the plasmacytic reactions in the MC of tumour-draining lymph nodes are chiefly determined by effects (stimulating or suppressing) of the primary carcinomas. The topography of the lymph nodes, however, seems to be the main determinant influencing the PC content of MC.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00735029

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Sponge aggregation factor: In situ localization by fluorescent monoclonal antibody techniques (1986)

Gramzow, Monika ; Dorn, August ; Steffen, Renate ; [et al.]

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 31 (1986), S. 251-258

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ISSN: 0730-2312

Keywords: aggregation factor ; monoclonal antibodies ; reaggregation ; cell recognition ; Geodia cydonium ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Chemistry and Pharmacology , Medicine

Notes: The aggregation factor (AF) from sponges mediates a heterophilic interaction of homologous cells. Applying electron microscopical means, we succeeded only very rarely in identifying the 90 S AF particle in tissue sections from Geodia cydonium. By means of a fluorescent antibody technique, we have now localized the cell binding domain of the AF in situ. Previous studies in this laboratory have led to the identification of the 47-kDa cell binding protein of the AF, using the monoclonal antibody (mab) 5D2-D11 [Gramzow M, Bachmann M, Zahn RK, Uhlenbruck G, Dorn A, Müller WEG, J Cell Biol, 102:1344-1349, 1986]. This mab and mab 7D5, directed against a 92-kDa protein in the AF complex, were chosen for the fluorescent studies. By using mab 5D2-D11, the plasma membranes of cells from different regions in the sponge could be brightly stained. However, mab 7D5 reacted only very weakly with the sponge surfaces. By applying the immuno-blotting technique it was furthermore demonstrated that the cell binding protein is present both in the associated form with AF complex and in a free state. Moreover, it was established that the 47-kDa binding protein is not present in (1) homologous glycoconjugates, (2) lectin, or (3) collagen; these components are known to be involved in cell-matrix interaction.

Additional Material: 5 Ill.