ISSN:
1432-0533
Keywords:
Human pituitary gland
;
Tissue culture
;
Density gradients
;
Immunocytochemistry
;
Growth hormone
;
Prolactin
;
Adrenocorticotropin
;
Follicle-stimulating hormone
;
Luteinizing hormone
;
Thyrotropin
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Pituitary cells were isolated from adult human pituitary glands obtained at autopsy 4–12 h postmortem by enzyme treatment (collagenase and dispase) and by Percoll density gradients. Cells thus isolated were maintained in culture for more than 6 months. By immunoperoxidase staining methods using rabbit sera monospecific against various pituitary hormones, a large number of cultured cells reacted positively. The hormones identified in these cells were growth hormone, prolactin, adrenocorticotropin, follicle-stimulting hormone, luteinizing hormone and thyrotropin. Electron-microscopic examination of cultured cells revealed the presence of secretory granules in cytoplasm characteristic of in vivo human pituitary cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00690196
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