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  • 1985-1989  (2)
  • Ca current  (1)
  • Growth rate  (1)
  • Catecholamines
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 410 (1987), S. 385-393 
    ISSN: 1432-2013
    Keywords: Bovine aortic endothelial cells ; Patch clamp ; Inward rectifier ; Transient outward K current ; Ca current ; Endothelium-derived relaxing factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The electrophysiological properties of cultured bovine aortic endothelial cells were characterized using the patch clamp technique. Resting potentials were measured on passing to the whole cell recording configuration and were close to −65 mV in healthy cells. In cell-attached recordings with a high potassium pipette solution, inward single channel currents were observed with zero applied pipette potential. A linear slope conductance of 25 pS was found for a wide range of hyperpolarizing patch potentials and also for depolarizing patch potentials of up to 50–60 mV. A pronounced inward rectification was apparent as no reversal of these currents was seen for larger depolarizations. Whole cell recording in physiological solutions revealed the presence of a hyperpolarization-activated inward current with strong inward rectification and no voltage-dependent ionic current was observed upon depolarization in this subset of cells. Substitution of potassium for external sodium resulted in a shift in the zero current potential consistent with potassium being the main permeant ion. Together with the characteristic voltage-dependent blocking actions of external sodium ions and low concentrations of barium and caesium ions, our results indicate that this current is very similar to the classical inward rectifier as originally described in skeletal muscle and in tunicate eggs. In a second population of cells, a depolarization-activated outward current displaying characteristics of the fast transient A-type potassium current as first reported in molluscan neurones was also observed. No evidence for inward voltage dependent sodium or calcium currents was found.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Oats ; Selection ; Growth rate ; Biological yield ; Independent culling ; Harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Grain yield was selected in Avena sativa X A. sterilis populations of segregates by applying independent culling for harvest index and either vegetative growth index or unit straw weight. Samples of lines intensively selected for harvest index had high harvest index but low grain yield because they had low vigor. Populations intensively selected for growth rate index or unit straw weight had high biological yield but low harvest index. Intensive selection for grain yield per se resulted in samples with high grain yield, but they were late and tall. Samples selected for harvest index at a 25% selection intensity first and subsequently selected for vegetative growth index or unit straw weight had grain yield as high as the samples selected for grain yield per se and vegetative growth index, and they had acceptable heading date and plant height. Backcrosses three and four were best, among the various BC generations, for selecting oat lines with high grain yield and suitable agronomic traits. CI 7463 was superior to CI 8044 as a recurrent parent, and B 445 was inferior to other A. sterilis accessions as a donor parent.
    Type of Medium: Electronic Resource
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