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  • 1985-1989  (2)
  • Capsella (Brassicaceae)  (1)
  • DNA sequence  (1)
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  • 1985-1989  (2)
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Aspartate aminotransferase isozymes in the genusCapsella (Brassicaceae): Subcellular location, gene duplication, and polymorphism (1989)
    Springer
    Biochemical genetics 27 (1989), S. 77-90 
    Details
    ISSN: 1573-4927
    Keywords: aspartate aminotransferase ; allozymes ; plastid enzymes ; Capsella (Brassicaceae) ; polyploidization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The subcellular location of aspartate aminotransferase isozymes (EC 2.6.1.1) in the genusCapsella(Brassicaceae) was studied. The diploid speciesC. grandiflora andC. rubella have three AAT isozymes, including one located in the plastids. Each locus is duplicated in the tetraploidCapsella bursa-pastoris. Variation at the plastid-coding locus exceeded that at the other loci.C. bursa-pastoris had some unique alleles not detected in the diploid species. Segregation in open-pollinated families revealed thatCapsella grandiflora was outcrossing, whereasC. rubella was highly inbred, with most populations homozygous or uniform at all three loci. Inheritance in the tetraploid colonizerC. bursa-pastoris is disomic. This species was also predominantly selfing with outcrossing rates between 2% and 10%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00563019
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The origin of transfer (oriT) of the conjugative plasmid R46: Characterization by deletion analysis and DNA sequencing (1987)
    Springer
    Molecular genetics and genomics 208 (1987), S. 219-225 
    Details
    ISSN: 1617-4623
    Keywords: Bacterial conjugation ; oriT ; DNA sequence ; Tn1725 mutagenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The origin of transfer (oriT) is the sequence within which conjugal transfer of plasmid DNA is initiated, and is absolutely required in cis for plasmid mobilization. We have cloned oriT from the 52 kb IncN plasmid R46 on a 600 bp fragment, and mapped the limits of the relevant sequence by deletion analysis and transposon mutagenesis. The nucleotide sequence of the oriT region contains 13 direct repeats of an 11 bp consensus sequence, 3 different pairs of 10 bp inverted repeats, and a segment that is extremely A-T rich. The direct repeats are within a region required for high frequency transfer and their sequence is such that their periodic alignment along the helix may induce curvature of the DNA. Analysis of Tn1725 insertions within the sequenced fragment of R46 revealed that, unlike most other transposons, transposition of Tn1725 can cause target sequence duplications of three different sizes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330445
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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