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  • 1
    Electronic Resource
    Electronic Resource
    Separation of rabbit epiphyseal chondrocytes in various stages of differentiation (1988)
    Springer
    Cell & tissue research 254 (1988), S. 393-398 
    Details
    ISSN: 1432-0878
    Keywords: Epiphyseal chondrocytes ; Differentiation ; Cell separation ; Cell culture ; Matrix synthesis ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Separation of fractions enriched in hypertrophic cells and proliferative cells has been achieved by density gradient centrifugation of cells from collagenase digests of rabbit epiphyseal cartilage. Concentrated suspensions of cells are centrifuged on a continuous Percoll density gradient. Hypertrophic cells remain in the upper part of the gradient and proliferative zone cells move to the lower regions. The resultant fractions show differences in mean cell diameter, alkaline phosphatase activity, morphology and synthetic activity in culture. Fractions rich in hypertrophic cells contain larger cells and more alkaline phosphatase activity than those enriched in proliferative cells. In culture the hypertrophic cells flatten as large irregular polygonal cells, whereas proliferative fractions form smaller spindle-shaped cells. In micromass culture hypertrophic fractions incorporate less 35S-sulphate and 14C-proline, and less tritiated thymidine than do proliferative fractions. These results suggest a general reduction in matrix and DNA synthesis with the attainment of the fully differentiated hypertrophic state, coincident with the expression of alkaline phosphatase activity and mineralisation of the cartilage matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225811
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Microvasculature of the pineal organ of the rainbow trout (Salmo gairdneri) (1987)
    Springer
    Cell & tissue research 250 (1987), S. 425-429 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Angioarchitecture (capillaries,arteries, veins) ; Corrosion-cast preparation method ; Scanning electron microscopy ; Salmo gairdneri
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The angioarchitecture of the pineal organ of the rainbow trout (Salmo gairdneri) was investigated by means of the corrosion-cast preparation method and scanning electron microscopy. Two main arteries (aa. epiphyseales) supply the pineal parenchyma. They emerge from the aa. cerebri anteriores and run in the fissure between the prosencephalon and the mesencephalon. After entering the pineal stalk, the aa. epiphyseales branch off into several arterioles, most of which extend to the pineal end-vesicle where they give rise to a lobular, bilaterally symmetric capillary network. Capillaries establishing the main portion of the pineal vessels appear widened in comparison to those supplying other portions of the brain and resemble capillaries in other endocrine organs. In Salmo gairdneri, no specialized system of portal vessels appears to exist between the pineal organ and other portions of the brain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219087
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    Paper (German National Licenses)
    Fulltext
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