ISSN:
0021-8995
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
,
Physics
Notes:
Macroporous hydrophilic poly-vinylpyridine beads were prepared by suspension copolymerization of 4- (or 2-)vinylpyridine and hydrophilic cross-linking agents, such as ethylene glycol dimethacrylate (1EG) and tetraethylene glycol dimethacrylate (4EG), and their physical properties were studied. Immobilized enzymes were obtained by adsorption of urease on vinylpyridine polymers and commercially available hydrophobic polymers. The poly-4-vinylpyridine resin crosslinked with 4EG (degree of crosslinkage, 25%) showed higher water regain, larger average pore radius, and the highest adsorption of urease. Leakage of urease from immobilized urease was scarcely observed in the buffer solution used. The hydrophilicity and hydrophobicity of support affected markedly the enzymatic reaction of the immobilized enzyme. As one of the applications of the polymer-supported urease, blood urea nitrogen in human sera was determined.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/app.1986.070320515
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