ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Chemically bonded phases
;
Off-line prepurification
;
Urinary 5-hydroxyindolacetic acid
;
Electrochemical detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Determination of serotonin (5-hydroxytryptamine, 5-HT) and its main metabolite, 5-hydroxyindolacetic acid (5-HIAA), became important in clinical chemistry. Commercially available and newly synthetised bonded-phase materials were evaluated in the off-line clean-up procedure of urine samples prior to the determination of 5-HIAA by high-performance liquid chromatography using electrochemical detection. From the point of reproducibility and recovery the best results were obtained employing our newly synthetised material consisting of silica gel support with wide pore diameter (≥20 nm) and large pore volume (≥2.5 cm3 g−1) with a mean (B.E.T.) surface area of 196m2 g−1. It has been modified by mixed chemically bonded C16−C18 phase. The achieved surface carbon coverage was 14.7% C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268778
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