ISSN:
1617-4623
Keywords:
cos
;
DNA gyrase
;
DNA packaging
;
Integration host factor
;
Terminase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A mutant of λ was isolated that grows in the Escherichia coli himAΔ/gyrB-him320(Ts) double mutant at 42°C; conditions which are non-permissive for wild-type λ growth. The responsible mutation, ohm1, alters the 40th codon of the Nul reading frame. The Nul and A gene products comprise the terminase protein which cleaves concatameric DNA into unit-length phage genomes during DNA packaging. The Nul-ohm1 gene product acts in trans to support λ growth in the double himA/gyrB mutant, and λcos154 growth in the single himA mutant. The observation that an alteration in Nul suppresses the inhibition of growth in the double himA/gyrB mutant implicates DNA gyrase, as well as integration host factor, in the DNA: protein interactions that occur at the initiation of packaging.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00322458
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