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  • 1985-1989  (2)
  • Endothelial cells  (1)
  • Peritoneo-venöser Shunt  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 245 (1986), S. 445-451 
    ISSN: 1432-0878
    Keywords: Endothelial cells ; ELISA ; Immunofluorescence ; Interspecific antigen recognition ; Serum-free culture ; Amphibian cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary AHG-associated protein (AHG-a.p.), the antigen of the blood-clotting factor VIII complex, is a specific endothelial cell marker. Primary (p-XTH) and established (XTH-2) endothelial cells from the hearts of Xenopus laevis tadpoles were assayed for the presence of this marker by means of immunological cross-reaction (recognition of common antigenic sites) with antiserum against human AHG-a.p. Radial imtnunodiffusion and rocket immunoelectrophoresis proved to be insufficiently sensitive, whereas immunofluorescence and a newly evaluated ELISA technique gave positive results. The very high sensitivity of the ELISA (less than 1/240000 of the AHG-a.p. in 0.1 ml human standard plasma can be detected) and the removal of interfering proteins by gel filtration also revealed the presence of AHG-a.p. in the fetal calf serum used in the culture medium; earlier investigations into this subject by a one-step radioimmunoassay had reported negative results. Specially adapted XTH-2 cells were grown in a proteinand serum-free hydrolysate medium in order to demonstrate the presence of a Xenopus-derived antigen that was immunoreactive with the anti-human AHG-a.p.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-2451
    Keywords: Peritoneovenous shunts ; Implantation technique ; Long-term results ; Peritoneo-venöser Shunt ; Implantationstechnik ; Langzeitergebnis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der peritoneo-venöse Shunt (PVS) hat sich in den letzten Jahren in der Therapie des therapieresistenten Ascites bewährt. Im Film werden Indikationsstellung, aktuelle Shuntsysteme, Implantationstechnik, Komplikationen, postop. Verlauf and Langzeitergebnisse dargestellt. Von 1976–1985 wurden bei 61 Patienten insgesamt 69 Shuntsysteme implantiert. Häufigste Ursache war die Lebercirrhose. Durchschnittliche Funktionsdauer betragt 21,7 Monate, längste Implantationsdauer 7 Jahre. Die Komplikationsrate ist erheblich: 19 Patienten verstarben wahrend des stationären Aufenthaltes. Gefürchteste Komplikationen sind die Shuntinfektion and Gerinnungsstörung. Trotz der hohen Komplikationsrate ist die Anlage eines PVS zu empfehlen.
    Notes: Summary Implantation of a peritoneovenous shunt has proven effective in the treatment of therapyresistant ascites. The film shows indication, present shunt systems, implantation technique, complications, postoperative course, and long-term results. From 1976 to 1985, a total of 68 shunt systems were implanted in 61 patients. The most common cause was hepatic cirrhosis. The average functional period is 21.7 months and the longest implantation time nearly 7 years. The complication rate is considerable: 19 patients died during hospitalization. The most dreaded complications are shunt infection and coagulation disturbances. In spite of the high complication rate, our experience shows that the peritoneovenous shunt can be recommended for combatting therapy-resistant ascites.
    Type of Medium: Electronic Resource
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