ISSN:
1420-9071
Keywords:
Enzyme immunoassay
;
peptide
;
kassinin
;
biotinylation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary A novel and sensitive noncompetitive enzyme immunoassay for kassinin is described. Kassinin was biotinylated using sulfosuccinimidyl-6-(biotinamido)hexanoate. The biotinylated kassinin was trapped on antikassinin IgG-coated polystyrene balls and, after washing to eliminate other biotinylated substances, was eluted with HCl. The biotinylated kassinin eluted was reacted with anti-kassinin Fab'-peroxidase conjugate and trapped onto streptavidin-coated polystyrene balls. Peroxidase activity bound to the polystyrene balls was assayed by fluorimetry. The detection limit of kassinin was 0.13 pg (0.1 fmol)/tube or 0.065 μg/l of rat plasma, which was 750-fold or 15-fold lower than that for competitive radioimmunoassay.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01952033
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