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  • 1985-1989  (4)
  • electron microscopy  (2)
  • Gaschromatography  (1)
  • Hymenoptera  (1)
Source
Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Passive smoking under controlled conditions (1985)
    Springer
    International archives of occupational and environmental health 56 (1985), S. 99-110 
    Details
    ISSN: 1432-1246
    Keywords: Identical exposure ; Nicotine ; Cotinine ; Saliva ; Urine ; RIA ; Gaschromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ten healthy subjects were exposed to passive smoking at a high level corresponding to 25–30 ppm CO in the ambient air for 3 h. All subjects were exposed at the same time in a climatic chamber especially designed for exposure experiments. Despite an identical exposure rate considerable interindividual variability of subsequent nicotine and cotinine levels in saliva, plasma and 24-h urine were observed. This variability was more prominent in nicotine than in cotinine levels. The kinetic pattern as reflected by saliva levels for up to 24 h was consistent with previous data found in active smokers. Nicotine levels found in saliva were markedly influenced by repeated sampling. This was not the case for cotinine levels. With regard to laboratory techniques RIA seems to be more sensitive than gaschromatography (GC). The results of this study suggest that measuring cotinine levels in 24-h urine with RIA is presently the most sensitive and reliable criterion for estimating exposure to passive smoking and for validating questionnaires or interviews about short-term exposure to passive smoking.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00379381
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Alarm substances of the stingless bee,Trigona silvestriana (1985)
    Springer
    Journal of chemical ecology 11 (1985), S. 409-416 
    Details
    ISSN: 1573-1561
    Keywords: Alarm substances ; nest defense ; 2-heptanol ; 2-nonanol ; mandibular gland ; Hymenoptera ; Apidae ; Meliponinae ; stingless bees ; Trigona silvestriana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract 2-Nonanol, 2-heptanol, octyl decanoate, and octyl octanoate were identified from the heads ofTrigona silvestriana workers. When presented at the nest, 2-nonanol, 2-heptanol, and the mixture of the four compounds elicited angular flights, landing, and buzzing of guard bees. Octyl octanoate elicited a weaker response. No response was given to octyl decanoate, to the ether solvent, or to the control volatile, vanillin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00989552
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Attempted sexing of bovine spermatozoa by fractionation on a Percoll density gradient (1988)
    New York, NY : Wiley-Blackwell
    Gamete Research 20 (1988), S. 83-92 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoal ; separation ; flow cytometry ; semen sexing ; electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Bovine spermatozoa were fractionated on Percoll density gradients into two major subpopulations of motile spermatozoa and a minor fraction containing mostly nonmotile spermatozoa with abnormal morphology. Fractionation required the addition of bovine serum albumin and a continuous Percoll gradient buffered with sodium bicarbonate. It is postulated that, under suitable ionic conditions, the binding of bovine serum albumin to spermatozoa amplifies subtle differences between subpopulations. These studies were directed toward separating Y- and X-bearing spermatozoa. However, when the subpopulations were evaluated by flow cytometry, their Y:X ratios were similar to that of an unfractionated control.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120200108
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The morphological and ultrastructural appearance of the crater defect in stallion spermatozoa (1985)
    New York, NY : Wiley-Blackwell
    Gamete Research 12 (1985), S. 41-46 
    Details
    ISSN: 0148-7280
    Keywords: spermatozoa ; stallion ; crater defect ; electron microscopy ; nuclear ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The appearance of the nuclear abnormality “crater defect” is described in spermatozoa from the domestic horse (Equadae). Under scanning electron microscopy, the defect appears as a severe depression at any area of the sperm nucleus or as a blister or swelling at some point on the sperm nucleus. Ultrastructurally, the crater appears as a nuclear vacuole containing amorphous material similar to that described in bull and boar sperm. The craters ranged in size from 0.5 to 2 μm in diameter. Within ejaculates of stallions having this defect, the percentages of sperm with crater defects varied widely over time, being as high as 60% and as low as 15%.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120120105
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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