ISSN:
1432-2242
Keywords:
Gliadins
;
Glutenins
;
DNA sequence
;
Genes
;
Gli-1 locus
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A cDNA clone (pTag1436) carrying a complete coding sequence for a γ-gliadin polypeptide has been identified and sequenced. By hybridisation to size fractionated poly A+ RNA from wheat nullisomic-tetrasomic lines, homologous transcripts from the Gli-A1, Gli-B1 and Gli-D1 loci were identified. These mRNAs differed from those complementary to a low molecular weight (LMW) glutenin cDNA clone. Hybridization of pTag1436 to digested wheat DNA produced a pattern of fragments unrelated to that obtained using a LMW glutenin cDNA probe. These results indicate that the γ-gliadin and LMW glutenin families, although both located at the Gli-1 loci, are distinct by hybridisation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00266556
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