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  • 1
    Electronic Resource
    Electronic Resource
    Stable transformation of maize: the impact of feeder cells on protoplast growth and transformation efficiency (1989)
    Springer
    Plant cell reports 8 (1989), S. 292-295 
    Details
    ISSN: 1432-203X
    Keywords: Zea mays L. ; Transformation ; Protoplast ; Kanamycin ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The importance of cell culture conditions, including the use of feeder cells, on protoplast growth and transformation in maize (Zea mays L.) was investigated. Total GUS activity, measured two days after transformation, was five-fold higher in protoplasts cultured on feeder cells compared to those grown in the absence of feeder cells. Since the specific activity of GUS was only slightly higher in the transformed protoplasts plated over feeder cells, the stimulation in transient gene expression resulted mainly from the improved environment provided by the feeder system. For stable transformation, either PEG treatment or electroporation of protoplasts was used to introduce the neo gene. When PEG was used, over 85% of the putative transformants (resistant to kanamycin) contained the neo gene. The combination of PEG transformation and the optimized cell culture protocol using feeder cells enabled the selection of about 100 stably transformed lines per gFW of cells. Electroporation was less efficient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274133
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  • 2
    Electronic Resource
    Electronic Resource
    Nest plundering allomones of the fire beeTrigona (Oxytrigona) mellicolor (1988)
    Springer
    Journal of chemical ecology 14 (1988), S. 495-501 
    Details
    ISSN: 1573-1561
    Keywords: Fire bee ; Trigona (Oxtrigona) mellicolor ; Trigona (Oxtrigona) tataira ; honeybee ; Apis mellifera ; Hymenoptera ; Apidae ; mandibular gland secretion ; allomone ; nest plundering ; diketones ; (E)-3-heptene-2,5-dione ; (E)-3-nonene-2,5-dione
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ten volatile compounds derived from the cephalic glands of the fire beeTrigona (Oxytrigona)mellicolor were bioassayed for possible allomonal activities facilitating nest plundering. Two diketones, (E)-3-heptene-2,5-dione and (E)-3-nonene-2,5-dione, caused the honeybeeApis mellifera to display avoidance behavior and reduced defensive behavior. These diketones are produced in relatively large quantities in fire-bee cephalic glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01013901
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    Paper (German National Licenses)
    Fulltext
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