ISSN:
0192-253X
Keywords:
Drosophila melanogaster
;
Yolk protein
;
Alcohol dehydrogenase
;
Life and Medical Sciences
;
Genetics
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
In Drosophila melanogaster the yolk protein (YP) genes are normally expressed only in the fat body and follicular epithelium of adult females-never in males or in larvae. We describe here a first step toward a genetic examination of the developmental controls that restrict the activity of the YP genes to adult female tissues. A YP1 promoter that contains the tissue-, temporal-, and sex-specific controlling elements for expression was fused to the reporter gene, alcohol dehy-drogenase (Adh). The gene fusion was transformed into an Adh-deficient genotype. As assayed by a number of criteria, that the fusion gene is expressed in the same physiological manner as the endogenous yolk protein genes. The fusion gene's activity is modulated in trans by a temperature-sensitive allele of the sex determination gene, tra-2. The Adh enzyme serves as a selectable marker and therefore these flies are suitable for use in genetic screens for trons-acting mutations that affect the expression of the yolk protein genes.
Additional Material:
8 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/dvg.1020100105
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