ISSN:
1432-0983
Keywords:
Double-stranded RNA
;
dsRNA
;
Mitochondria
;
Stem rusts
;
Leaf rusts
;
Cereals
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Double-stranded ribonucleic acids (dsRNA) were isolated from mitochondria in urediosporelings of three cereal stem rusts, Puccinia graminis f. sp. tritici, f. sp. secalis and f. sp. avenae, and two cereal leaf rusts, P. recondita f. sp. tritici and P. coronata f. sp. avenae, and analyzed by agarose gel electrophoresis. The double strandedness of the RNA molecules was characterized by nuclease treatments (RNase A, DNase 1 and S1 nuclease) and CF-11 cellulose column chromatography. No interspecific variation in multisegments of dsRNA was observed among races of each forma specialis. As to the interspecific variation, although each of three forma specialis of Puccinia graminis had similar dsRNA segments, 4.8, 5.0 and 5.2 kb, wheat leaf rust and oat crown rust had additional dsRNA segments of 2.7, 2.8, 5.8 and 6.0 kb. The presence of a dsRNA segment of 5 kb size in all isolates and species examined indicates that this unique segment can be a molecular marker for the rust family, Uredinales. Dot-blot hybridization indicated that there is no sequence homology between dsRNA segments and mitochondrial DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00435464
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