ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Phenylthiocarbamyl amino acids
;
Pre-column derivatization
;
Gas-phase protein hydrolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary An HPLC system has been developed in which 23 phenylthiocarbamyl (PTC) amino acids are distinctly separated. Applying this system, the preceding steps in HPLC amino acid analysis i.e. gas-phase hydrolysis of the protein/peptide sample and the pre-column derivatization, were evaluated and optimized. PTC amino acids in HPLC buffer solution are stable for at least 16 hours if stored at 5°–8°C. The sensitivity of the method is in the low pmol range.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268788
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