ISSN:
1573-5044
Keywords:
Mucuna pruriens
;
entrapped plant cells
;
bioconversions
;
catechols
;
isolation
;
mass spectrometry
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Alginate-entrapped cells ofM. pruriens were able to convert a number of parasubstituted monophenolic compounds into the corresponding catechols. All catechols produced were released into the medium, which offered the opportunity to isolate these products via a relatively simple procedure. Prepurification was performed on a Sephadex G10 gel and catechols were concentrated on Affigel 601. The identity of all products was confirmed with combined liquid chromatography/mass spectrometry (LC/MS) or MS using the desorption chemical ionization technique, depending on the catechol. For the entrapped cells and for a cell homogenate prepared of the same cell line ofM. pruriens the substrate specificities were qualitatively identical when judged on initial rates of synthesis calculated on protein basis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00043043
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