ISSN:
1435-1536
Keywords:
Polyoxyethylene cholesterylethers
;
hemolysis
;
bovineerythrocyte
;
criticalmicelle concentration
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
,
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Abstract Hemolytic activity of nonionic surfactants, polyoxyethylene cholesteryl ethers, C27H45O(CH2CH2O) n H (Chol-E n ,n=, 25, 30, 50) and polyoxyethylene dihydrocholeseryl ethers, C27H47O(CH2CH2O) n H (DHChol-E n ,n=15, 30 50) were measured, changing the concentration of surfactant and erythrocyte at 37 °C. Maximum hemolytic activity was observed in these cholesteryl derivatives with 25–30 oxyethylene units. The time course of hemolysis was also measured as a function of the concentrations of surfactant and erythrocyte. Hemolysis started after a certain induction period,τ, and then apparently proceeded as a first-order reaction with respect to the erythrocyte concentration. The surfactant inducing 50% hemolysis at low concentration had a smallτ value and large rate constant. The maximum amount of adsorption without inducing hemolysis,a 0, decreased with increasing polyoxyethylene chain length. Chol-E25 has the maximum activity for the solubilization of egg yolk lecithin at 37 °C. Based on these results, the mechanism of hemolysis by these surfactants was quantitatively discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01412395
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