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Digitale Medien

Fusion polypeptides in gene cloning: Potential problems due to conformational alterations at the junction (1987)

Querol, E. ; Parrilla, A.

Springer

Cellular and molecular life sciences 43 (1987), S. 920-922

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Details

ISSN: 1420-9071

Schlagwort(e): Molecular cloning ; fusion polypeptides ; protein conformation ; protein folding

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin

Notizen: Summary Many eukaryotic genes are cloned in bacterial hosts as fusion polypeptides. Prediction of the secondary structures for some common prokaryotic fusion polypeptides shows that many junction sites correspond to important secondary structures. It is suggested that such structures could affect (hinder, etc.) the conformation or drive the folding of the neighboring eukaryotic counterparts. Thus the prokaryotic junction should be better performed, in random coil regions, or short fusion prokaryotic polypeptides should be sued.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01951668

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