ISSN:
1432-0428
Keywords:
Insulin
;
C-peptide
;
radio-immunoassay
;
haemolysis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Venous blood was taken at the end of a glucose infusion test in 19 individuals and divided into four aliquots, 3 of which were variably haemolysed by repeated passage through a 23-gauge needle to simulate traumatic venepuncture. Plasma insulin (measured by both a charcoal separation and a double-antibody method), C-peptide, and haemoglobin were measured in each aliquot, and haemolysis was also assessed visibly. A significant loss of immuno-assayable plasma insulin was found in samples with only a trace of visible haemolysis, with up to 90% lost in severely haemolysed samples. Plasma C-peptide was unaffected by haemolysis. This represents an additional advantage for the use of plasma C-peptide in assessing insulin secretion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00292540
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