ISSN:
1573-6903
Keywords:
GABA
;
cell cultures
;
ontogenetic development
;
cerebral cortex
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract High-affinity uptake of [3H]γ-aminobutyric acid (GABA) was studied in cultures of neonatal rat cortical neurons grown on pre-formed monolayers of non-neuronal (glial) cells. Both the maximum rate (V max) and, to a smaller extent, theK m of [3H]GABA uptake increased with time. In addition, in parallel with these changes, 2,4-diaminobutyric acid and cis-3-aminocyclohexane-1-carboxylic acid (ACHC), compounds which are considered typical substrate/inhibitors of GABA uptake in neurons, became progressively stronger inhibitors of [3H]GABA uptake. Consequently, the present results may mean that the studies using uptake, of [3H]GABA, [3H]ACHC, or [3H]DABA as a specific marker for GABAergic neurons differentiating during the ontogenetic development of the central nervous system may have to be interpreted with caution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00971315
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