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  • 1
    Electronic Resource
    Electronic Resource
    In Vitro Response to Fusarium Elicitor and Toxic Substances in Crosses between Resistant and Susceptible Carnation Cultivars (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 98 (1987), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: To assess the correlation between in vivo resistance to F. oxysporum f. sp. dianthi and in vitro behaviour, calli from several resistant and susceptible Mediterranean carnation cultivars and F1 progenies of crosses were tested both for the ability to grow on a medium containing crude culture filtrate of F. oxysporum and to produce phytoalexins following treatment with cell wall components (elicitor) of the same fungus. The results show no significant correlation between in vivo resistance and in vitro tolerance to culture filtrate of the fungus, while there was a good correlation in die case of phytoalexin production. Moreover, the character phytoalexin production behaved as a dominant in the crosses between a resistant and a susceptible cultivar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1987.tb01141.x
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  • 2
    Electronic Resource
    Electronic Resource
    Inheritance of solar direction finding in sandhoppers (1985)
    Springer
    Journal of comparative physiology 157 (1985), S. 433-440 
    Details
    ISSN: 1432-1351
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three natural populations ofTalitrus saltator living on differently orientated seashores and the offspring produced by crossing two of them were analysed by means of progeny tests, with the aim of gaining some data on the genetic control of solar direction finding. Data from fullsib family-analyses showed a genetic heterogeneity within at least two of the populations tested: some families oriented towards the sea, some in a different direction and others were dispersed. Intrafamily behaviour in the first and second generations and a mass analysis of dispersed families, suggests that dispersion may arise from segregation of heterozygous parental genotypes. The data obtained generally support the hypothesis of an oligogenic rather than a polygenic control of direction finding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00615143
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Correlations between in vivo resistance to Fusarium and in vitro response to fungal elicitors and toxic substances in carnation (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 42-47 
    Details
    ISSN: 1432-2242
    Keywords: Dianthus caryophyllus ; Fusarium oxysporum ; Physiological resistance genetics ; Tissue cultures ; Phytoalexin metabolism ; Toxins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary With the aim of ascertaining the existance of a correlation between in vivo resistance to Fusarium oxysporum f. sp. dianthi and in vitro response to fungal elicitors and toxic substances, phenylalanine ammonialyase and phytoalexin accumulation, on one hand, and resistance to culture filtrate, on the other, were assayed in “in vitro” cultures of three susceptible and four resistant Dianthus caryophyllus cultivars. Cultivars showing varying degrees of resistance in vivo either tolerated higher culture filtrate concentrations (‘Niki’) or showed high PAL activity and phytoalexin production when treated with Fusarium elicitor (‘Duca’), or responded positively to both treatments (‘Mei-Ling’, ‘Pulcino’). No such responses were shown in tissue cultures of susceptible cultivars. The differential response to the fungal elicitor seemed to be highly specific as genetic differences between cultivars were not observed in tissue cultures treated with other biotic (Phytophthora infestans) and abiotic (HgCl2) elicitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264480
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation of tomato cell lines with altered response to Fusarium cell wall components (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 37-40 
    Details
    ISSN: 1432-2242
    Keywords: Elicitor ; Fusarium oxysporum ; in vitro selection ; Pathogen resistance ; Phytoalexin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To obtain Tomato cell lines with an altered capacity to respond to heat-released cell wall components (elicitor) of a tomato pathogen (Fusarium oxysporum f. sp. lycopersici), positive and negative selection experiments, using BUdR enrichment techniques, were carried out on suspension cultures of the susceptible, low phytoalexin producer cultivar Red River. Both high and low phytoalexin producing clones were isolated. Further tests demonstrated that not all phytoalexin-producing clones were more susceptible to the elicitor toxic effect, and that they were altered also in the speed of response to fungal cell wall components. Cells selected with Fusarium elicitor showed the same behaviour when challenged by Phytophthora infestans elicitor, thus suggesting in this case lack of specificity. The results are finally discussed with a view to using the technique both as a tool to study the genetics and physiology of hostparasite interactions and as a possible new method for the selection of pathogen resistant genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249139
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  • 5
    Electronic Resource
    Electronic Resource
    The pleiotropic phenotype of tomato cells selected for altered response to Fusarium oxysporium f. sp. lycopersici cell wall components (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 689-695 
    Details
    ISSN: 1432-2242
    Keywords: Lycopersicon aesculentum ; Fusarium oxysporum ; Peroxidases ; Ion leakage ; Esopolysaccharides ; Dual cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary With the aim of better understanding in vitro host-parasite interactions, tomato cell lines selected for altered response to Fusarium oxysporum f. sp. lycopersici cell wall components were further characterized. Particularly, their behaviour in dual culture in regard to both fungal inhibition and peroxidase activation was analysed and selected, and control cell clones were screened for esopolysaccharide content and toxin tolerance. Interclonal differences in growth response to 2,4-D and DMSO and the capacity to grow on a medium devoid of hormones (habituation) were taken as parameters representative of physiological variability not directly correlated with the response to pathogens. Significant differences between clones selected for increased (F+) and decreased (F−) response to fungal elicitors were found for pathogen inhibition, peroxidase and esopolysaccharide content, toxin tolerance being reduced in F but not significantly different from the control in F+. As expected, clonal variability for the response to 2,4-D and DMSO, although significant, was not connected with hostparasite interactions. The data reported thus show that selection for a character (response to elicitors), probably critical for the response to pathogens, may lead to the recovery of genotypes showing a set of modifications suggestive of a cascade of events leading to active defense.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262565
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  • 6
    Electronic Resource
    Electronic Resource
    Interspecific differences in differentiation and dedifferentiation patterns in the genusNicotiana (1985)
    Springer
    Plant systematics and evolution 151 (1985), S. 19-29 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Solanaceae ; Nicotiana. — Habituation ; differentiation ; crown gall ; tissue culture ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differentiation on hormoneless media, habituation ability and crown gall induction inNicotiana tissue cultures have been used as physiological parameters of evolutionary differentiation between species. Some of them on hormone free media differentiated whole plantlets, others produced only shoots or roots or showed undifferentiated growth (habituation), some eventually died. Moreover, the same genotypes showed a differential behaviour as far as tumor formation byAgrobacterium tumefaciens was concerned. Particularly, the competence for crown gall transformation inNicotiana species seems negatively correlated with differentiation capacity and may be ascribed to differences in the plants capacity to synthesize growth regulators. The correlation between the results obtained and the phylogenetic position of the genotypes tested is finally discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02418016
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  • 7
    Electronic Resource
    Electronic Resource
    Tumor formation and morphogenesis on different Nicotiana sp and hybrids induced by Agrobacterium tumefaciens T-DNA mutants (1987)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 8 (1987), S. 61-71 
    Details
    ISSN: 0192-253X
    Keywords: Agrobacterium insertion mutants ; hormone equilibria ; differentiation ; dedifferentiation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A series of experiments are presented that have been performed to observe the interactions between Agrobacterium tumefaciens strains mutated in the T-DNA genes involved in indoleacetic acid and cytokinin biosynthesis and several Nicotiana species and hybrids. Infections were induced on leaf cuttings of Nicotiana debneyi, N. knightiana, N. clevelandii, N. bigelovii var bigelovii, N. bigelovii var quadrivalvis, N. glauca, N. langsdorffii, the amphidiploid tumorous hybrid N. glauca × N. langsdorffii, and a nontumorous mutant of it. The effect of deletions of the Ti plasmid varied according to plant genotype. Insertion mutants in iaaM and iaaH suppressed tumor formation in N. langsdorffii, reduced it in N. bigeloviivar quadrivalvis, had no effect in N. glauca and the two amphidiploid hybrids, and promoted tumorigenesis when compared to the wild-type Agrobacterium strain B6S3 in N. bigelovii N. debneyi, and N. knightiana. The same mutations induced shoot formation in N. glauca, increased it in N. debneyi, and suppressed root formation in N. knightiana. On the other hand, an insertion mutation of the isopentenyl transferase gene (ipt-) had no effect in N. bigelovii var quadrivalvis, N. debneyi, the tumorous hybrid, suppressed tumor formation in N. langsdorffii, and inhibited it in N. glauca, the nontumorous hybrid, N. bigelovii var bigelovii, and N. knightiana. Insertion in ipt suppressed shoot formation in the nontumorous hybrid and inhibited it in the nontumorous amphidiploid and N. debneyi, while promoting root formation in N. glauca and N. debneyi.The suggestion of the existence of specific hormone equilibria necessary for the shift to each morphogenetic pattern was supported by experiments with exogenous hormone treatments of three genotypes (N. glauca, N. langsdorffii, and the nontumorous N. glauca × N. langsdorffii).
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020080202
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