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  • 1985-1989  (4)
  • 1
    Electronic Resource
    Electronic Resource
    An improved method for mass rearing Paratheresia claripalpis for biological control of lepidopteran stalk borers (1989)
    Springer
    Entomologia experimentalis et applicata 51 (1989), S. 37-40 
    Details
    ISSN: 1570-7458
    Keywords: biological control ; mass rearing ; Paratheresia claripalpis ; stalk borer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Une technique d'élevage de massa de la tachinaire, P. claripalpis Van der Wulp, est décrite et comparée à la technique classique. Cette technique correspond à une amélioration puisqu'elle demande moins de travail et produit 2.25 fois plus de parasites pour le même nombre d'hôtes.
    Notes: Abstract An improved method for mass rearing Paratheresia claripalpis Van der Wulp (Diptera: Tachinidae), for biological control of lepidopteran stalk borers is described and compared to the commonly used method. The improved method was found to be superior by being less labour intensive and by producing 2.25 times more parasites from the same number of hosts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343441
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of three rabbit rotavirus strains (1988)
    Springer
    Archives of virology 98 (1988), S. 253-265 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We report biochemical (RNA and protein patterns and gene-coding assignments) and serologic (serotype and subgroup) properties of three strains of rabbit rotaviruses — Ala, C11 and R2. The RNA electropherotypes were a standard “short” pattern for R2, an unusual “short” pattern for Ala, and an unusual “long” pattern for C11. Serologic studies indicated that these viruses were all group A serotype 3 rotaviruses. In addition, the Ala and C11 viruses were found to possess subgroup I specificity, whereas the R2 virus possessed subgroup II specificity. In contrast to their distinctive RNA patterns, the polypeptide patterns of the rabbit viruses were similar to those of SA11. To identify cognate genes and determine gene-coding assignments for the rabbit rotaviruses, cDNA probes of individual SA11 genes were hybridized to rabbit rotaviral genomic RNA segments that had been separated by polyacrylamide gel electrophoresis and transferred to filters (Northern blots). The order of genome segments 7–11 for each of the rabbit rotaviruses was unique and differed from that of SA11 genes. These differences were possibly due to rearrangements of the RNA sequences within these individual genome segments. Sequence analysis of the individual RNA segments will confirm whether genome rearrangements are the molecular basis for these novel migration patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322173
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Minimal infective dose of rotavirus (1987)
    Springer
    Archives of virology 92 (1987), S. 261-271 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We studied the minimal infective dose of the gastroenteritis virus, rotavirus. Increasingly lower doses [104, 103, 101, 1, 10−2 plaque forming units (PFU)] of the OSU strain of porcine rotavirus were administered to highly susceptible (colostrum deprived, cesarean derived) newborn miniature swine piglets.In vitro studies showed that virus infectivity was inactivated in piglet gastric juice, both by low pH and by pH- and concentration-dependent factor(s). These factors remain unidentified, but to prevent intragastric viral inactivation, sodium bicarbonate was administered prior to oral virus inoculation of piglets with virulent (non-tissue culture passaged) virus. The lowest dose of virus to induce clinical illness or to demonstrate viral replication by recovery of significantly more infectious virus than was administered, or both, was 1 PFU. These results should help establish standards for virus contamination of water and recommendations for evaluating disinfection procedures for rotaviruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317483
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Influence of media supplements on growth and survival ofCampylobacter pylori (1989)
    Springer
    European journal of clinical microbiology & infectious diseases 8 (1989), S. 597-602 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Experiments were designed to determine the role of heme and the importance of other factors in the growth ofCampylobacter pylori. Campylobacter pylori strains were tested for their ability to synthesize porphyrin, for their ability to grow and be maintained on basal medium and basal medium supplemented with blood or blood products, and for the influence of bovine serum albumin and catalase on viability. Results indicated thatCampylobacter pylori does not require heme as a source of porphyrin. Growth ofCampylobacter pylori could not be sustained on media containing starch or hemoglobin, but was sustained on media containing erythrocytes, serum, bovine serum albumin or catalase. The ability to grow on media containing bovine serum albumin and catalase suggests that protection from toxic fatty acids and the prevention of toxic product formation may be important factors in the growth and survival ofCampylobacter pylori in vitro. Both bovine serum albumin and catalase combined provide the minimum requirements which allow the spectrum ofCampylobacter pylori present in a single culture to grow on blood-free media.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968136
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    Paper (German National Licenses)
    Fulltext
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