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  • 1
    Electronic Resource
    Electronic Resource
    Growth kinetics of the Golgi apparatus during the cell cycle in onion root meristems (1988)
    Springer
    Planta 175 (1988), S. 305-312 
    Details
    ISSN: 1432-2048
    Keywords: Allium ; Cell cycle ; Golgi apparatus ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In onion root meristems, the number of dictyosomes per cell shows a kinetics of growth strongly related to the cell cycle. During the interphase of steady-state proliferative cells, the volume density and numerical density of the Golgi apparatus decrease to reach minimum values in late-interphase cells, characterized by their greatest length. This pattern is also found in the total volume occupied by Golgi apparatus. Once in mitosis, the above-mentioned parameters begin to increase reaching maximum mean values in telophase. After the experimental uncoupling of chromosome and growth cycles by presynchronization with hydroxyurea, we found a similar behaviour pattern in the Golgi apparatus: decreasing values during interphase and a triggering of Golgi-apparatus growth in prophase independently of the bigger cell sizes reached in mitosis as an effect of pretreatment with hydroxyurea. These results indicate a cyclic kinetics of this subcellular component in higher-plant meristems, coupled with early mitotic events.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396335
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunocytochemical and morphometrical study of thyrotropic cells of Rana ridibunda (1988)
    Springer
    Histochemistry and cell biology 89 (1988), S. 461-466 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Indirect immunoflorescence and PAP techniques for light microscopy as well as the immunogold complex technique for electron microscopy were used to localize and identify thyrotropic (TSH) producing cells in the pars distalis of Rana ridibunda. A double immunostaining procedure was used to distinguish TSH cells from other glycoprotein hormone producing cells. Rabbit anti-human-β-TSH was used as the primary antiserum and revealed a basophil, PAS and alcian blue positive cell type in the ventro-central zone of the gland. Under the electron microscope, TSH cells show irregular morphology, polymorphic secretory granules with diameters ranging between 120 and 375 nm and poor development of the endoplasmic reticulum and Golgi complex; they are usually polarized towards capillaries. Ultrastructural morphometry (point-counting method) was used to evaluate stereological parameters of rough endoplasmic reticulum, Golgi complex, secretory granules and mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492603
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Changes of dictyosome ultrastructure during the cell cycle in onion root meristematic cells. A morphometric and stereological study (1988)
    Springer
    Protoplasma 146 (1988), S. 35-40 
    Details
    ISSN: 1615-6102
    Keywords: Cell cycle ; Dictyosome ultrastructure ; Quantitative analysis ; Root meristems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dictyosome ultrastructure changes during the cell cycle in onion root meristematic cells. Changes were mainly related to cisternae and intercisternal spaces morphology. Taking each dictyosome to be composed of three different regions (CIS, medial, and TRANS), several quantitative changes were detected in some of the compartments. Many of the planimetric parameters evaluated showed higher values in medical cisternae, while CIS and TRANS remained nearly constant. We have also found an increased activity of dictyosomes, as indicated by increase in the volume fraction of vesicular attached structures. This reaches maximum values at ana-telophase in coincidence with the onset and progression of cytokinesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01354293
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    Paper (German National Licenses)
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