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  • 1985-1989  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Detection of antigen-specific IgE-plaque-forming cells from peripheral-blood lymphocytes of ragweed- and grass-allergic patients (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 15 (1985), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The reverse haemolytic plaque assay was developed to measure antigen (AgE or rye grass I)-specific IgE-plaque-forming cells from the peripheral-blood lymphocytes of ragweed- and grass-allergic patients. The anti-IgE-developing antisera was shown to be isotype-specific, and the response of the assay was inhibited by 52% by the addition of 10 pg of antigen. In addition, the assay was shown to have a reproducibility (s.d.) of 15%. The blood lymphocytes from all fifteen atopic (grass and ragweed) patients were shown to form antigen-specific IgE-plaque-forming cells during the pollen season (mean value 115 cells) and up to 6 months after the season (mean value 56 cells). Cycloheximide appears to block the formation of the plaque-forming cells. This method appears to be sensitive and reproducible enough to study in vitro IgE antibody synthesis of peripheral-blood lymphocytes from atopies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1985.tb02254.x
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of antigen stimulation on antigen-specific IgE-plaque-forming cells from peripheral-blood lymphocytes of atopics (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 15 (1985), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In the present study, it was shown that allergen challenge in vitro produced an increase in the number of antigen-specific IgE-plaque-forming cells of peripheral-blood lymphocytes from grass- or ragweed-allergic patients. Thus, the blood lymphocytes of all twelve (four rye grass I and eight AgE) sensitive donors responded whereas the blood lymphocytes of five non-atopic controls were unresponsive to antigen challenge. Allergen challenge doses of 10−10−10−12 g/ml were found to give the greatest number of plaque-forming cells whereas the number of plaque-forming cells at challenging doses between 10−9 and 10−7 g/ml were either the same or less than those obtained with unchallenged cells. The results are discussed as to whether this in vitro model system represents in vivo response to allergen of the allergic patient.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1985.tb02253.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Lymphocyte Subpopulations in Extrinsic Allergic Alveolitis (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 465 (1986), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1986.tb18512.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of In Vitro Proteolysis on the Allergenicity of Major Whey Proteins (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 54 (1989), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of in vitro proteolysis on the allergenicity of major whey proteins (α-lactalbumin and β-lactoglobulin) by simulating the human gastrointestinal conditions were evaluated. The proteolysis of demineralized whey with pepsin (pH 2.0 for 30 min) and/or various pancreatic enzymes (pH 7.5 for 60 and 240 min) was performed by the pH-stat technique at 37°C. The enzyme inactivation was performed by heating at 80°C for 20 min. Allergenicity of the hydrolyzates was evaluated by RAST inhibition using sera obtained from children allergic to whey proteins. Selective proteolysis of whey by pepsin and α-chymotrypsin was the most efficient combination of enzymes to reduce the allergenicity of both α-lactalbumin and β-lactoglobulin. The above hydrolyzate could be used to develop an ingredient for infant milk formula with a lower allergenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1989.tb07938.x
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Immunogenicity and Allergenicity of Whey Protein Hydrolysates (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Allergic reactions to cow's milk are common in children. The sera of allergic subjects have been found to contain specific IgE antibodies against several whey proteins such as α-lactalbumin (α-la) and β-lactoglobulin (β-lg). This work aims to study the effect of hydrolysis on the immunogenicity and the allergenicity of α-la and β-lg. Whey proteins were submitted to hydrolysis with immobilized α-chymotrypsin. High performance liquid chromatography analysis showed a decrease of native whey proteins and the appearance of low molecular weight peptides. The immunogenicity of these hydrolysates was not modified, but their allergenicity, as assessed by RAST inhibition, was decreased. The results may have important clinical implications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb13563.x
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    Paper (German National Licenses)
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