ISSN:
1432-0428
Keywords:
Sorbitol
;
myoinositol
;
endothelial cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Cultured human endothelial cells were incubated with high glucose (27.5 mmol/l) supplemented medium for 0, 2, 6, 12, 24 and 48 h. Lactate dehydrogenase was assayed as a measure of cell number. Sorbitol and myoinositol were determined as trimethyl silyl derivatives using gas chromatography mass spectrometry. Sorbitol, after an initial lag phase of 2 h, accumulated rapidly to plateau at 2 to 3 fold control value by 24 h, no further increase being seen over a further 24 h (Baseline, 1.31±0.36, 24 h 3.50±0.74 pmol sorbitol/ unit lactate dehydrogenase; p〈0.001). There was no change in myoinositol levels over the 24 h period. Sorbitol accumulation occurred at glucose concentration 〉18.1 mmol/l and only returned to baseline levels after 24 h of incubation in normal medium (glucose levels 5.9 mmol/l). Sorbitol levels are increased by incubation of endothelial cells at high glucose levels but myoinositol levels remain unchanged.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00265088
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