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  • 1985-1989  (6)
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  • 1
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies against Vibrio salmonicida: the causative agent of coldwater vibriosis (‘Hitra disease’) in Atlantic salmon, Salmo salar L. (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 11 (1988), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. Monoclonal antibodies (Mab) directed against Vibrio salmonicida were produced and partially characterized. The bacterium is the causative agent of ‘Hitra disease’ or cotdwater vibriosis (CV) and differs from all other Vibrio bacteria tested so far with respect to a unique surface antigen (VS-P1). Thirteen hybridoma clones produced antibodies which exclusively reacted with this antigen in ELISA. The remaining four clones reacted against undefined determinants and were partly cross-reactive to V. anguiilarum, V. ordalii and V. fischeri. Fifteen Mab were of IgG1/kappa and two of the IgG3/kappa isotypes. Eleven of the IgG1 plus the two IgG3 Mab reacted with the VS-P1 molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1988.tb00541.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a surface layer antigen from Vibrio salmonicida (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of fish diseases 11 (1988), S. 0 
    Details
    ISSN: 1365-2761
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: Abstract. A cell surface product (VS-P1) of Vibrio salmonicida has been purified from culture supernatant by a combination of extensive dialysis, filtration and centrifugation, as well as by salt precipitation and hydrophobic chromatography. SDS-PAGE analysis showed that the monomeric form of the antigen is a single polypeptide with an apparent molecular weight of 40000. Size exclusion HPLC of purified VS-P1 as well as VS-Pl-containing fish serum revealed, however, oligomeric forms in the range from 300000 to more than 700000 daltons. The antigen contained 6% carbohydrate and several isolectric forms were distinguishable when analysed on an analytical isoelectric focusing electrophoresis system. A ‘sandwich’ ELISA, utilizing polyclonal antibodies, was developed for screening sera from both healthy and moribund Atlantic salmon for the presence of the VS-P1 antigen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2761.1988.tb00540.x
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Variations in the content of trypsin and trysinogen in larval herring (Clupea harengus) digesting copepod nauplii (1987)
    Springer
    Marine biology 94 (1987), S. 171-181 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Trypsin and its proform trypsinogen were quantified by radioimmunoassay in herring (Clupea harengus L.) larvae subjected to different prey densities. During the first weeks of larval life, the enzyme content fluctuated in a threephased pattern. Yolk resorption (Phase 1) was characterized by an increase in enzyme. During the first few days after yolk resorption (Phase 2), there was a sharp decline in enzyme. Older larvae (Phase 3) exhibited a second period of intensive enzyme synthesis. Amounts of trypsin in intestines of feeding larvae were analysed. At first feeding, a basal level of gut enzyme of approximately 30ng was recorded, and the amount of additional enzyme secreted from the pancreatic tissue into the intestine appeared to be dependent upon the numbers of prey items ingested. The enzyme-substrate ratio in the intestine was approximately 1 to 4. Prey availability affected amount of trypsinogen. Larvae experiencing a high prey density had an approximately two-fold higher specific enzyme content in Phase 2 compared to larvae exposed to a low prey density. A proposed nutritional strategy for first feeding herring larvae is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00392929
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Fate of trypsin and assimilation efficiency in larval herring (Clupea harengus) following digestion of copepods (1988)
    Springer
    Marine biology 97 (1988), S. 467-476 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fate of the protease trypsin in intestines of individual herring larvae Clupea harengus L. was studied following digestion of the copepod Acartia tonsa. Trypsin was retained in the intestine during two consecutive pulses of feeding and defaecation of copepods. Quantification of herring trypsin in digested, defaecated copepods showed that ca. 1% of larval intestinal enzyme was defaecated along with 1 to 3 copepods. Following ingestion of a single meal, the level of intestinal trypsin post-ingestion declined to pre-ingestion levels within 1 to 2 d of starvation. All enzyme data thus indicated that trypsin, released in response to ingestion of a meal, was retained. In addition, analysis of fed subgroups of starved larvae clearly indicated that release of trypsin from the pancreas stopped after 6 to 8 d of starvation. As the fish still contained substantial amounts of trypsinogen, the underlying cause might be defective release mechanisms. Daily secretion of trypsin and processes responsible for enzyme retention in the gut are discussed. Assimilation efficiency in herring larvae was estimated for copepodite prey. Average carbon assimilation was 90%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391042
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Trypsin content in intestines of herring larvae, Clupea harengus, ingesting inert polystyrene spheres or live crustacea prey (1988)
    Springer
    Marine biology 98 (1988), S. 331-335 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In 1986, at the Danish Institute of Fisheries and Marine Research, Denmark, Clupea harengus L. larvae from three different herring stocks were offered either non-biodegradable polystyrene spheres, nauplii and copepodites of Acartia tonsa or Artemia ssp. nauplii. Ingestion of polystyrene spheres induced trypsin secretion to a higher level than in non-feeding fish. Larvae ingesting live food of the same width as the polystyrene spheres exhibited the highest trypsin content in the intestines. Mechanisms responsible for the regulation of pancreatic enzyme secretion are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391108
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Degradation of human epidermal keratin by cod trypsin and extracts of fish intestines (1989)
    Springer
    Archives of dermatological research 280 (1989), S. 469-473 
    Details
    ISSN: 1432-069X
    Keywords: Epidermal keratin ; Fish trypsin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cod Gadus morhua and bovine trypsin degraded human epidermal keratin with similar efficacies in vitro around optimal pH, which was at pH 8.4 for cod trypsin and at pH 9.5 for bovine trypsin. Extract of intestines of cod, Atlantic herring Clupea harengus, Atlantic salmon Salmo salar, and redfish Sebastes marinus degraded keratin with similar efficacies with pH optima between 8.5 and 9.5. Sheets of plantar callus were degraded with somewhat lower efficacy than keratin. The keratin-degrading activity of extract of cod intestines had a temperature optimum around 45°C. Inhibition with benzamidine and 4-phenylbutylamine showed that trypsin amounted to more than 2/3 of the keratin-degrading activity in all extracts of fish intestines. Apart from cod intestines, which had the lowest chymotrypsin content, chymotrypsin made a smaller but significant contribution to the keratin-degrading activity. The present investigation demonstrates that fish trypsin and extract of fish intestines are effective in degrading human epidermal keratin in vitro, and in a recent investigation the same was shown with fish pepsin. This may suggest a possible mechanism for the development of irritative contact eczema caused by exposure to fish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427658
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    Paper (German National Licenses)
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