ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Highly acidic proteins are difficult to study using conventional electrofocusing and electrophoretic techniques. For example, the lysosomal enzymes from the cellular slime mold Dictyostelium discoideum have isoelectric points (pI) around 3-4 due to extensive phosphorylation and sulfation. To assess differences in anionic modifications among these lysosomal enzymes, we required methods that resolved acidic proteins on the basis of their net charge. The separation procedures needed to be rapid and convenient, allowing parallel analysis of a large number of samples. Using these criteria, we developed two methods, isoelectric focusing in horizontal agarose gels and native electrophoresis in vertical polyacrylamide gels, which are more useful than other available techniques. Since the mechanism by which each system separates proteins is different, both methods give important information about acidic proteins.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150070904
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