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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 28 (1988), S. 345-349 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In a newly constructed one-vessel dialysis fermentor, a strain of Staphylococcus carnosus TM300 carrying the lipase secretion plasmid pLipPS1 was used to investigate exoenzyme and biomass production. The bacterial culture grows in an inner compartment of 21 volume, separated from a 101 nutrient broth compartment by a conventional dialysis membrane. In order to avoid substrate depletion and to prolong the growth phase, a highly concentrated nutrient broth was used. The biomass production reached 60 g cell dry weight/l. The increase in extracellular lipase concentration was directly coupled with the increase of cell mass and reached a value of 230 mg/l culture supernatant. Harvesting the cells in the late growth phase, the lipase content was about 30% of the total exoproteins in the supernatant.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 534-541 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Perstraction (membrane-aided solvent extraction) was utilized for elimination of ethanol inhibition in continuous ethanol fermentation using high sugar concentrate. Hollow fibers for an artificial kidney were used as a permeable membrane, and their capacity to extract ethanol was examined by using several organic solvents. When tri-n-butylphosphate was used as an extractant, a 500 g/L feed glucose medium was successfully fermented by immobilized yeast cells. During this continuous fermentation a high ehtanol productivity of 48 g/h-L-gel was held, and the solvent requirement per consumed glucose was 6 L-solvent/kg-glucose.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0009-286X
    Keywords: Biogas ; Methan-Produktion ; anaerobe Abfallbehandlung ; zweistufiges Verfahren ; Essigsäure-Kinetik ; Pilzmycel ; Penicillin ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Chemie Ingenieur Technik - CIT 59 (1987), S. 907-917 
    ISSN: 0009-286X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Hydrodynamic stress resistance of microorganisms. The production of microbial metabolites and the growth of microorganisms are affected by mechanical forces, such as occur on stirring, pumping, and aeration in industrial equipment. This paper considers the methods of investigation and typical experimental results. The sensitivity of the organisms depends upon the stability of the cell wall and upon its morphology. Raising the viscosity of nutrient solution increases the symptoms of damage at a given stirrer speed. In the case of methane-producing microorganisms, growth and product formation (methane production) are affected in the same manner. Physiological stress, induced for example by the absence of essential nutrients, proves additive to mechanical stress in its effect. It is suggested that the power dissipation per unit volume should be taken as a guide on scale-up or on transfer of experience gained with one kind of flow system (e. g. jet flow) to another (e. g. stirred tank).
    Additional Material: 37 Ill.
    Type of Medium: Electronic Resource
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