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  • 1985-1989  (1)
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    Electronic Resource
    Electronic Resource
    Mechanism and optimized conditions for PEG mediated DNA transfection into plant protoplasts (1989)
    Springer
    Plant cell reports 8 (1989), S. 148-151 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Experimental conditions influencing DNA uptake efficiency by maize protoplasts in polyethyleneglycol (PEG) mediated transfection experiments have been studied systematically. The data provide evidence that the extracellular DNA is precipitated efficiently by combined action of PEG together with divalent cations and DNA is taken up by the plant protoplasts in the precipitated form. The particle size is strongly effected by the pH of the PEG solution. At optimal pH 6– 6.5 a very fine and homogenous precipitate forms in presence of Ca2+ and Mg2+ ions and is efficiently incorporated by maize and rice protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716828
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