ISSN:
1572-8773
Keywords:
Nitrous oxide respiration
;
Copper chromophore biosynthesis
;
Cytochromeed 1
;
Denitrification
;
Periplasmic enzyme
;
Pseudomonas
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Copper-deficient cells ofPseudomonas stutzeri strain ZoBell synthesize catalytically inactive nitrous oxide (N2O) reductase which is activated by added Cu(II) in the absence of de novo protein synthesis. The apparentK m for the activation process is 0.13 μM. Activation is temperature-dependent and is inhibited by Cd(II)(K i 1.27 μM) and less strongly by Zn(II), Ni(II), and Co(II). The same metal ions at 20 μM have little or no effect on N2O reduction of intact cells. Apo-N2O reductase of transposon Tn5-inducednos − mutants with defective Cu-chromophore biosynthesis is not reactivated by Cu(II). N2O reductase of Cu-sufficient and Cu-deficient wild type, and ofnos − mutants is localized in the periplasm, the latter providing the likely site of metal incorporation into the apoenzyme.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01138070
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